CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010721
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ephrin-B1 
Protein Synonyms/Alias
 EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2 
Gene Name
 EFNB1 
Gene Synonyms/Alias
 EFL3; EPLG2; LERK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
289LSTLASPKGGSGTAGubiquitination[1, 2, 3, 4, 5]
345SPANIYYKV******ubiquitination[1, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity). 
Sequence Annotation
 DOMAIN 30 164 Ephrin RBD.
 MOTIF 344 346 PDZ-binding (Potential).
 MOD_RES 287 287 Phosphoserine.
 CARBOHYD 139 139 N-linked (GlcNAc...) (Potential).
 DISULFID 64 101 By similarity.
 DISULFID 89 153 By similarity.  
Keyword
 Complete proteome; Developmental protein; Differentiation; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; Membrane; Neurogenesis; Phosphoprotein; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 346 AA 
Protein Sequence
MARPGQRWLG KWLVAMVVWA LCRLATPLAK NLEPVSWSSL NPKFLSGKGL VIYPKIGDKL 60
DIICPRAEAG RPYEYYKLYL VRPEQAAACS TVLDPNVLVT CNRPEQEIRF TIKFQEFSPN 120
YMGLEFKKHH DYYITSTSNG SLEGLENREG GVCRTRTMKI IMKVGQDPNA VTPEQLTTSR 180
PSKEADNTVK MATQAPGSRG SLGDSDGKHE TVNQEEKSGP GASGGSSGDP DGFFNSKVAL 240
FAAVGAGCVI FLLIIIFLTV LLLKLRKRHR KHTQQRAAAL SLSTLASPKG GSGTAGTEPS 300
DIIIPLRTTE NNYCPHYEKV SGDYGHPVYI VQEMPPQSPA NIYYKV 346 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0045121; C:membrane raft; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0045202; C:synapse; ISS:UniProtKB.
 GO:0046875; F:ephrin receptor binding; TAS:ProtInc.
 GO:0007411; P:axon guidance; IEA:Compara.
 GO:0007155; P:cell adhesion; TAS:ProtInc.
 GO:0007267; P:cell-cell signaling; TAS:ProtInc.
 GO:0009880; P:embryonic pattern specification; IEA:Compara.
 GO:0001755; P:neural crest cell migration; IEA:Compara.
 GO:0042102; P:positive regulation of T cell proliferation; IEA:Compara. 
Interpro
 IPR008972; Cupredoxin.
 IPR001799; Ephrin.
 IPR019765; Ephrin_CS. 
Pfam
 PF00812; Ephrin 
SMART
  
PROSITE
 PS01299; EPHRIN_RBD_1
 PS51551; EPHRIN_RBD_2 
PRINTS
 PR01347; EPHRIN.