CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006859
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase CHK1 
Protein Synonyms/Alias
 Checkpoint kinase 1 
Gene Name
 CHK1 
Gene Synonyms/Alias
 YBR274W; YBR1742 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
144GVAHRDIKPENILLDubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit. 
Sequence Annotation
 DOMAIN 15 281 Protein kinase.
 NP_BIND 21 29 ATP (By similarity).
 ACT_SITE 142 142 Proton acceptor (By similarity).
 BINDING 45 45 ATP (By similarity).  
Keyword
 ATP-binding; Cell cycle; Complete proteome; DNA damage; Kinase; Nucleotide-binding; Nucleus; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 527 AA 
Protein Sequence
MSLSQVSPLP HIKDVVLGDT VGQGAFACVK NAHLQMDPSI ILAVKFIHVP TCKKMGLSDK 60
DITKEVVLQS KCSKHPNVLR LIDCNVSKEY MWIILEMADG GDLFDKIEPD VGVDSDVAQF 120
YFQQLVSAIN YLHVECGVAH RDIKPENILL DKNGNLKLAD FGLASQFRRK DGTLRVSMDQ 180
RGSPPYMAPE VLYSEEGYYA DRTDIWSIGI LLFVLLTGQT PWELPSLENE DFVFFIENDG 240
NLNWGPWSKI EFTHLNLLRK ILQPDPNKRV TLKALKLHPW VLRRASFSGD DGLCNDPELL 300
AKKLFSHLKV SLSNENYLKF TQDTNSNNRY ISTQPIGNEL AELEHDSMHF QTVSNTQRAF 360
TSYDSNTNYN SGTGMTQEAK WTQFISYDIA ALQFHSDEND CNELVKRHLQ FNPNKLTKFY 420
TLQPMDVLLP ILEKALNLSQ IRVKPDLFAN FERLCELLGY DNVFPLIINI KTKSNGGYQL 480
CGSISIIKIE EELKSVGFER KTGDPLEWRR LFKKISTICR DIILIPN 527 
Gene Ontology
 GO:0005634; C:nucleus; IPI:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:SGD.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0000077; P:DNA damage checkpoint; IMP:SGD.
 GO:0048478; P:replication fork protection; IGI:SGD. 
Interpro
 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS