UniProt Accession

 CNDP2_MOUSE; Q9D1A2; Q3TA80; Q3TI32; Q3U7B9; Q99PV1 

Genbank Protein ID

 AB046738; AK003779; AK151013; AK152729; AK168029; AK168652; AK172034; BC005532 

Genbank Nucleotide ID

 BAB21596.1; BAB22991.1; BAE30033.1; BAE31451.1; BAE40014.1; BAE40509.1; BAE42789.1; AAH05532.1 

Protein Name

 Cytosolic non-specific dipeptidase 

Protein Synonyms/Alias

 CNDP dipeptidase 2; Glutamate carboxypeptidase-like protein 1 

Gene Name

 Cndp2 

Gene Synonyms/Alias

 Cn2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
5	***MSALKAVFQYID	ubiquitination	[1]
20	ENQDRYVKKLAEWVA	ubiquitination	[1]
68	LVDIGKQKLPDGSEI	ubiquitination	[1]
301	ETLLHSCKKDILMHR	ubiquitination	[1]
339	IPRKVVGKFSIRLVP	ubiquitination	[1]
364	VSSYLSKKFAELQSP	acetylation	[2, 3]
364	VSSYLSKKFAELQSP	ubiquitination	[1]
402	QAGRRALKTVFGVEP	ubiquitination	[1]
430	TFQEATGKNVMLLPV	ubiquitination	[1]
450	GAHSQNEKLNRLNYI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Hydrolyzes a variety of dipeptides including L- carnosine. Has a strong preference for Cys-Gly (By similarity). 

Sequence Annotation

 REGION 166 167 Substrate binding.
 ACT_SITE 101 101 By similarity.
 ACT_SITE 166 166 Proton acceptor (By similarity).
 METAL 99 99 Manganese 2.
 METAL 132 132 Manganese 1.
 METAL 132 132 Manganese 2.
 METAL 167 167 Manganese 1.
 METAL 195 195 Manganese 2.
 METAL 445 445 Manganese 1.
 BINDING 195 195 Substrate; via carbonyl oxygen.
 BINDING 228 228 Substrate; shared with homodimeric
 BINDING 330 330 Substrate; shared with homodimeric
 BINDING 343 343 Substrate.
 BINDING 417 417 Substrate; via amide nitrogen and
 BINDING 445 445 Substrate.  

Keyword

 3D-structure; Carboxypeptidase; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Manganese; Metal-binding; Metalloprotease; Protease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 475 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISA:MGI.
 GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
 GO:0016805; F:dipeptidase activity; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
 GO:0034701; F:tripeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IDA:MGI. 

Interpro

 IPR001261; ArgE/DapE_CS.
 IPR017153; GSH_degradosome_DUG1.
 IPR002933; Peptidase_M20.
 IPR011650; Peptidase_M20_dimer. 

Pfam

 PF07687; M20_dimer
 PF01546; Peptidase_M20 

SMART

  

PROSITE

 PS00758; ARGE_DAPE_CPG2_1
 PS00759; ARGE_DAPE_CPG2_2 

PRINTS