UniProt Accession

 F261_RAT; P07953; P16119 

Genbank Protein ID

 Y00702; X15579; X15580; M26215; M26216; J04197; M27886 

Genbank Nucleotide ID

 CAA68694.1; CAA33606.1; CAA33607.1; AAA02888.2; AAA02889.1; AAA79008.1; AAA58780.1 

Protein Name

 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 

Protein Synonyms/Alias

 6PF-2-K/Fru-2,6-P2ase 1; PFK/FBPase 1; 6PF-2-K/Fru-2,6-P2ase liver isozyme; 6-phosphofructo-2-kinase; Fructose-2,6-bisphosphatase 

Gene Name

 Pfkfb1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
148	SLILQFAKEHGYKVF	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Synthesis and degradation of fructose 2,6-bisphosphate. 

Sequence Annotation

 NP_BIND 49 56 ATP (By similarity).
 REGION 2 250 6-phosphofructo-2-kinase.
 REGION 251 471 Fructose-2,6-bisphosphatase.
 ACT_SITE 131 131 Potential.
 ACT_SITE 161 161 Potential.
 ACT_SITE 259 259 Tele-phosphohistidine intermediate.
 ACT_SITE 328 328 Potential.
 ACT_SITE 393 393 Proton donor.
 BINDING 105 105 Fructose-6-phosphate (By similarity).
 BINDING 196 196 Fructose-6-phosphate (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 33 33 Phosphoserine; by PKA.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Direct protein sequencing; Hydrolase; Kinase; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 471 AA 

Protein Sequence

Gene Ontology

 GO:0043540; C:6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 complex; ISS:UniProtKB.
 GO:0003873; F:6-phosphofructo-2-kinase activity; IDA:RGD.
 GO:0005524; F:ATP binding; IDA:RGD.
 GO:0004331; F:fructose-2,6-bisphosphate 2-phosphatase activity; ISS:UniProtKB.
 GO:0070095; F:fructose-6-phosphate binding; IDA:RGD.
 GO:0042802; F:identical protein binding; IDA:RGD.
 GO:0006003; P:fructose 2,6-bisphosphate metabolic process; ISS:UniProtKB.
 GO:0006000; P:fructose metabolic process; IEA:InterPro.
 GO:0031100; P:organ regeneration; IEP:RGD.
 GO:0033133; P:positive regulation of glucokinase activity; IMP:RGD.
 GO:0051591; P:response to cAMP; IEP:RGD.
 GO:0033762; P:response to glucagon stimulus; IEP:RGD.
 GO:0051384; P:response to glucocorticoid stimulus; IEP:RGD.
 GO:0032868; P:response to insulin stimulus; IEP:RGD.
 GO:0042594; P:response to starvation; IEP:RGD. 

Interpro

 IPR003094; 6Pfruct_kin.
 IPR013079; 6Phosfructo_kin.
 IPR016260; Bifunct_6PFK/fruc_bisP_Ptase.
 IPR013078; His_Pase_superF_clade-1.
 IPR027417; P-loop_NTPase.
 IPR001345; PG/BPGM_mutase_AS. 

Pfam

 PF01591; 6PF2K
 PF00300; His_Phos_1 

SMART

 SM00855; PGAM 

PROSITE

 PS00175; PG_MUTASE 

PRINTS

 PR00991; 6PFRUCTKNASE.