CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009333
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein max 
Protein Synonyms/Alias
 Class D basic helix-loop-helix protein 4; bHLHd4; Myc-associated factor X 
Gene Name
 MAX 
Gene Synonyms/Alias
 BHLHD4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40RKRRDHIKDSFHSLRacetylation[1]
57VPSLQGEKASRAQILubiquitination[2]
66SRAQILDKATEYIQYacetylation[1]
153PEEPQSRKKLRMEASacetylation[1]
154EEPQSRKKLRMEAS*acetylation[1]
Reference
 [1] Max is acetylated by p300 at several nuclear localization residues.
 Faiola F, Wu YT, Pan S, Zhang K, Farina A, Martinez E.
 Biochem J. 2007 May 1;403(3):397-407. [PMID: 17217336]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, whereas the MAD-MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. 
Sequence Annotation
 DOMAIN 23 74 bHLH.
 REGION 81 102 Leucine-zipper.
 MOTIF 152 156 Nuclear localization signal.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 66 66 N6-acetyllysine (Probable).
 MOD_RES 153 153 N6-acetyllysine.
 MOD_RES 154 154 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Activator; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 160 AA 
Protein Sequence
MSDNDDIEVE SDEEQPRFQS AADKRAHHNA LERKRRDHIK DSFHSLRDSV PSLQGEKASR 60
AQILDKATEY IQYMRRKNHT HQQDIDDLKR QNALLEQQVR ALEKARSSAQ LQTNYPSSDN 120
SLYTNAKGST ISAFDGGSDS SSESEPEEPQ SRKKLRMEAS 160 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0071339; C:MLL1 complex; IDA:UniProtKB.
 GO:0016605; C:PML body; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0071375; P:cellular response to peptide hormone stimulus; IEA:Compara.
 GO:0009267; P:cellular response to starvation; IEA:Compara.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0051402; P:neuron apoptotic process; IEA:Compara.
 GO:0006461; P:protein complex assembly; IEA:Compara.
 GO:0048678; P:response to axon injury; IEA:Compara.
 GO:0032868; P:response to insulin stimulus; IEA:Compara.
 GO:0060041; P:retina development in camera-type eye; IEA:Compara.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR011598; bHLH_dom. 
Pfam
 PF00010; HLH 
SMART
 SM00353; HLH 
PROSITE
 PS50888; BHLH 
PRINTS