CPLM-010686

Genbank Nucleotide ID

Fumarate hydratase, mitochondrial

Protein Synonyms/Alias

Mus musculus (Mouse)

Position	Peptide	Type	References
58	FDTFGELKVPTDKYY	acetylation	[1, 2, 3]
58	FDTFGELKVPTDKYY	succinylation	[3]
58	FDTFGELKVPTDKYY	ubiquitination	[4]
63	ELKVPTDKYYGAQTV	acetylation	[1, 2, 3, 5, 6, 7, 8, 9, 10, 11]
63	ELKVPTDKYYGAQTV	succinylation	[3]
63	ELKVPTDKYYGAQTV	ubiquitination	[4]
77	VRSTMNFKIGGATER	acetylation	[1, 2, 3, 6, 8, 10, 11]
77	VRSTMNFKIGGATER	succinylation	[3]
77	VRSTMNFKIGGATER	ubiquitination	[4]
97	IQAFGILKRAAAEVN	acetylation	[8]
97	IQAFGILKRAAAEVN	ubiquitination	[4]
112	QEYGLDPKIASAIMK	acetylation	[1, 2, 3, 5, 6, 8, 9, 11]
112	QEYGLDPKIASAIMK	succinylation	[3]
112	QEYGLDPKIASAIMK	ubiquitination	[4]
119	KIASAIMKAADEVAE	acetylation	[1, 2, 3, 8, 11]
119	KIASAIMKAADEVAE	succinylation	[3]
119	KIASAIMKAADEVAE	ubiquitination	[4]
128	ADEVAEGKLNDHFPL	acetylation	[1]
170	GGELGSKKPVHPNDH	acetylation	[2, 8]
210	VLLPGLQKLHDALSA	acetylation	[1, 2, 3, 11]
210	VLLPGLQKLHDALSA	succinylation	[3]
220	DALSAKSKEFAQVIK	acetylation	[1, 2, 3, 11]
220	DALSAKSKEFAQVIK	succinylation	[3]
220	DALSAKSKEFAQVIK	ubiquitination	[4]
227	KEFAQVIKIGRTHTQ	acetylation	[8]
289	TRIGFAEKVAAKVAA	ubiquitination	[4]
293	FAEKVAAKVAALTGL	acetylation	[8]
474	KIAKTAHKNGSTLKE	acetylation	[5]

[1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[5] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
[6] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[7] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
[8] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[10] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
[11] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]

Functional Description

REGION 173 176 B site (By similarity).
REGION 183 185 Substrate binding (By similarity).
BINDING 144 144 Substrate (By similarity).
MOD_RES 63 63 N6-acetyllysine.
MOD_RES 77 77 N6-acetyllysine (By similarity).
MOD_RES 112 112 N6-acetyllysine.
MOD_RES 289 289 N6-acetyllysine (By similarity).
MOD_RES 474 474 N6-acetyllysine.

Acetylation; Alternative initiation; Complete proteome; Cytoplasm; Direct protein sequencing; Lyase; Mitochondrion; Reference proteome; Transit peptide; Tricarboxylic acid cycle.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; IDA:MGI.
GO:0045239; C:tricarboxylic acid cycle enzyme complex; IEA:InterPro.
GO:0004333; F:fumarate hydratase activity; IEA:EC.
GO:0006106; P:fumarate metabolic process; IEA:InterPro.
GO:0048873; P:homeostasis of number of cells within a tissue; IMP:MGI.
GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.

IPR005677; Fum_hydII.
IPR024083; Fumarase/histidase_N.
IPR018951; Fumarase_C_C.
IPR000362; Fumarate_lyase.
IPR020557; Fumarate_lyase_CS.
IPR022761; Fumarate_lyase_N.
IPR008948; L-Aspartase-like.

PF10415; FumaraseC_C
PF00206; Lyase_1

PS00163; FUMARATE_LYASES

PR00149; FUMRATELYASE.