CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030074
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pyruvate formate lyase I 
Protein Synonyms/Alias
  
Gene Name
 pflB 
Gene Synonyms/Alias
 ECDH10B_0973 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12 / DH10B) 
NCBI Taxa ID
 316385 
Lysine Modification
Position
Peptide
Type
References
63DKVMEGVKLENRTHAsuccinylation[1]
107LQTEAPLKRALIPFGsuccinylation[1]
117LIPFGGIKMIEGSCKsuccinylation[1]
124KMIEGSCKAYNRELDsuccinylation[1]
195YGIDYLMKDKLAQFTsuccinylation[1]
454INGGVDEKLKMQVGPsuccinylation[1]
467GPKSEPIKGDVLNYDsuccinylation[1]
654ASLTSVAKLPFAYAKsuccinylation[1]
Reference
 [1] Identification of lysine succinylation as a new post-translational modification.
 Zhang Z, Tan M, Xie Z, Dai L, Chen Y, Zhao Y.
 Nat Chem Biol. 2011 Jan;7(1):58-63. [PMID: 21151122] 
Functional Description
  
Sequence Annotation
 ACT_SITE 419 419 S-acetylcysteine intermediate (By
 ACT_SITE 420 420 Cysteine radical intermediate (By
 MOD_RES 735 735 Glycine radical (By similarity).  
Keyword
 Complete proteome; Lyase; Organic radical; Pyruvate. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 760 AA 
Protein Sequence
MSELNEKLAT AWEGFTKGDW QNEVNVRDFI QKNYTPYEGD ESFLAGATEA TTTLWDKVME 60
GVKLENRTHA PVDFDTAVAS TITSHDAGYI NKQLEKIVGL QTEAPLKRAL IPFGGIKMIE 120
GSCKAYNREL DPMIKKIFTE YRKTHNQGVF DVYTPDILRC RKSGVLTGLP DAYGRGRIIG 180
DYRRVALYGI DYLMKDKLAQ FTSLQADLEN GVNLEQTIRL REEIAEQHRA LGQMKEMAAK 240
YGYDISGPAT NAQEAIQWTY FGYLAAVKSQ NGAAMSFGRT STFLDVYIER DLKAGKITEQ 300
EAQEMVDHLV MKLRMVRFLR TPEYDELFSG DPIWATESIG GMGLDGRTLV TKNSFRFLNT 360
LYTMGPSPEP NMTILWSEKL PLNFKKFAAK VSIDTSSLQY ENDDLMRPDF NNDDYAIACC 420
VSPMIVGKQM QFFGARANLA KTMLYAINGG VDEKLKMQVG PKSEPIKGDV LNYDEVMERM 480
DHFMDWLAKQ YITALNIIHY MHDKYSYEAS LMALHDRDVI RTMACGIAGL SVAADSLSAI 540
KYAKVKPIRD EDGLAIDFEI EGEYPQFGNN DPRVDDLAVD LVERFMKKIQ KLHTYRDAIP 600
TQSVLTITSN VVYGKKTGNT PDGRRAGAPF GPGANPMHGR DQKGAVASLT SVAKLPFAYA 660
KDGISYTFSI VPNALGKDDE VRKTNLAGLM DGYFHHEASI EGGQHLNVNV MNREMLLDAM 720
ENPEKYPQLT IRVSGYAVRF NSLTKEQQQD VITRTFTQSM 760 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0008861; F:formate C-acetyltransferase activity; IEA:InterPro.
 GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
 GO:0005975; P:carbohydrate metabolic process; IEA:InterPro. 
Interpro
 IPR005949; Form_AcTrfase.
 IPR019777; Form_AcTrfase_GR_CS.
 IPR001150; Gly_radical.
 IPR004184; PFL_dom. 
Pfam
 PF01228; Gly_radical
 PF02901; PFL 
SMART
  
PROSITE
 PS00850; GLY_RADICAL_1
 PS51149; GLY_RADICAL_2
 PS51554; PFL 
PRINTS