CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012776
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heterogeneous nuclear ribonucleoprotein U-like protein 2 
Protein Synonyms/Alias
 Scaffold-attachment factor A2; SAF-A2 
Gene Name
 HNRNPUL2 
Gene Synonyms/Alias
 HNRPUL2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
174GSEVPGDKAAEEQGDubiquitination[1]
187GDDQDSEKSKPAGSDubiquitination[1]
273GQPLFSEKFPTLWSGubiquitination[2, 3]
291TYGVTKGKVCFEAKVubiquitination[4]
305VTQNLPMKEGCTEVSubiquitination[4]
406LLPHVLCKNCVVELNubiquitination[2, 3]
471GKTQWALKYAKENPEacetylation[5]
471GKTQWALKYAKENPEubiquitination[2, 3, 4]
496VLNQMRMKGLEEPEMubiquitination[4]
521QASQCLSKLVQIASRubiquitination[4, 6]
548YNSGQRRKLLLFKTFubiquitination[4]
553RRKLLLFKTFSRKVVubiquitination[2, 3, 4, 7]
601ANFSLPEKCDYMDEVubiquitination[4]
624EAQPIVTKYKEEARKubiquitination[2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
  
Sequence Annotation
 DOMAIN 3 37 SAP.
 DOMAIN 226 419 B30.2/SPRY.
 MOD_RES 161 161 Phosphoserine (By similarity).
 MOD_RES 188 188 Phosphoserine (By similarity).
 MOD_RES 228 228 Phosphoserine.  
Keyword
 Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 747 AA 
Protein Sequence
MEVKRLKVTE LRSELQRRGL DSRGLKVDLA QRLQEALDAE MLEDEAGGGG AGPGGACKAE 60
PRPVAASGGG PGGDEEEDEE EEEEDEEALL EDEDEEPPPA QALGQAAQPP PEPPEAAAME 120
AAAEPDASEK PAEATAGSGG VNGGEEQGLG KREEDEPEER SGDETPGSEV PGDKAAEEQG 180
DDQDSEKSKP AGSDGERRGV KRQRDEKDEH GRAYYEFREE AYHSRSKSPL PPEEEAKDEE 240
EDQTLVNLDT YTSDLHFQVS KDRYGGQPLF SEKFPTLWSG ARSTYGVTKG KVCFEAKVTQ 300
NLPMKEGCTE VSLLRVGWSV DFSRPQLGED EFSYGFDGRG LKAENGQFEE FGQTFGENDV 360
IGCFANFETE EVELSFSKNG EDLGVAFWIS KDSLADRALL PHVLCKNCVV ELNFGQKEEP 420
FFPPPEEFVF IHAVPVEERV RTAVPPKTIE ECEVILMVGL PGSGKTQWAL KYAKENPEKR 480
YNVLGAETVL NQMRMKGLEE PEMDPKSRDL LVQQASQCLS KLVQIASRTK RNFILDQCNV 540
YNSGQRRKLL LFKTFSRKVV VVVPNEEDWK KRLELRKEVE GDDVPESIML EMKANFSLPE 600
KCDYMDEVTY GELEKEEAQP IVTKYKEEAR KLLPPSEKRT NRRNNRNKRN RQNRSRGQGY 660
VGGQRRGYDN RAYGQQYWGQ PGNRGGYRNF YDRYRGDYDR FYGRDYEYNR YRDYYRQYNR 720
DWQSYYYHHP QDRDRYYRNY YGYQGYR 747 
Gene Ontology
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR008985; ConA-like_lec_gl_sf.
 IPR027026; hnRNP_U_like_2.
 IPR027417; P-loop_NTPase.
 IPR003034; SAP_dom.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 
Pfam
 PF02037; SAP
 PF00622; SPRY 
SMART
 SM00513; SAP
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY
 PS50800; SAP 
PRINTS