UniProt Accession

 SRP54_ECOLI; P0AGD7; P07019; P77007; P77008 

Genbank Protein ID

 X01818; U00096; AP009048 

Genbank Nucleotide ID

 CAA25957.1; AAC75659.1; BAA16495.2 

Protein Name

 Signal recognition particle protein 

Protein Synonyms/Alias

 Fifty-four homolog; Ffh; p48 

Gene Name

 ffh 

Gene Synonyms/Alias

 b2610; JW5414 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
28	RLTEDNVKDTLREVR	acetylation	[1]
66	AVGHEVNKSLTPGQE	acetylation	[1]
76	TPGQEFVKIVRNELV	acetylation	[1]
182	ALKEAKLKFYDVLLV	acetylation	[1]
278	KFLGVGEKTEALEPF	acetylation	[1]
318	VDRAQAEKLASKLKK	acetylation	[1]
371	VKSQMDDKVLVRMEA	acetylation	[1]
418	QDVNRLLKQFDDMQR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components. 

Sequence Annotation

 NP_BIND 107 114 GTP (By similarity).
 NP_BIND 190 194 GTP (By similarity).
 NP_BIND 248 251 GTP (By similarity).  

Keyword

 3D-structure; Complete proteome; Cytoplasm; GTP-binding; Nucleotide-binding; Reference proteome; Ribonucleoprotein; RNA-binding; Signal recognition particle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 453 AA 

Protein Sequence

Gene Ontology

 GO:0048500; C:signal recognition particle; IMP:EcoliWiki.
 GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IEA:UniProtKB-KW.
 GO:0008312; F:7S RNA binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IDA:EcoliWiki.
 GO:0003924; F:GTPase activity; IMP:EcoCyc.
 GO:0006612; P:protein targeting to membrane; IMP:EcoliWiki.
 GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR027417; P-loop_NTPase.
 IPR013822; Signal_recog_particl_SRP54_hlx.
 IPR004125; Signal_recog_particle_SRP54_M.
 IPR022941; SRP54.
 IPR000897; SRP54_GTPase_dom.
 IPR004780; SRP_Ffh. 

Pfam

 PF00448; SRP54
 PF02881; SRP54_N
 PF02978; SRP_SPB 

SMART

 SM00382; AAA
 SM00962; SRP54
 SM00963; SRP54_N 

PROSITE

 PS00300; SRP54 

PRINTS