UniProt Accession

 NEP1_YEAST; Q06287; D6VYI9; E9P8U2 

Genbank Protein ID

 U17246; AY557941; BK006945 

Genbank Nucleotide ID

 AAB67457.1; AAS56267.1; DAA09505.1 

Protein Name

 Ribosomal RNA small subunit methyltransferase NEP1 

Protein Synonyms/Alias

 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1; 18S rRNA Psi1189 methyltransferase; Essential for mitotic growth 1; Nucleolar essential protein 1 

Gene Name

 EMG1 

Gene Synonyms/Alias

 NEP1; YLR186W; L9470.5 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
147	LMVQLLHKLSIRSVN	methylation	[1]

Reference

 [1] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074] 

Functional Description

 Has an essential role in 40S ribosomal subunit biogenesis and 18S rRNA processing. Probably catalyzes the N1- methylation of pseudouridine at position 1189 (Psi1189) in 18S rRNA. Thus, is most likely the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3- carboxypropyl) pseudouridine (m1acp3-Psi) in position 1189 in 18S rRNA. Is also involved in ribosome assembly. 

Sequence Annotation

 REGION 212 214 S-adenosyl-L-methionine binding.
 REGION 227 232 S-adenosyl-L-methionine binding.
 BINDING 180 180 S-adenosyl-L-methionine; via carbonyl
 BINDING 207 207 S-adenosyl-L-methionine; via amide  

Keyword

 3D-structure; Complete proteome; Methyltransferase; Nucleus; Reference proteome; Ribonucleoprotein; Ribosome biogenesis; RNA-binding; rRNA processing; rRNA-binding; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 252 AA 

Protein Sequence

Gene Ontology

 GO:0030686; C:90S preribosome; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005880; C:nuclear microtubule; IDA:SGD.
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0032040; C:small-subunit processome; IDA:SGD.
 GO:0070037; F:rRNA (pseudouridine) methyltransferase activity; TAS:UniProtKB.
 GO:0019843; F:rRNA binding; IDA:UniProtKB.
 GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0070475; P:rRNA base methylation; IMP:SGD. 

Interpro

 IPR005304; Rbsml_bgen_MeTrfase_EMG1/NEP1. 

Pfam

 PF03587; EMG1 

SMART

  

PROSITE

  

PRINTS