CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037439
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 AP-2 complex subunit mu 
Protein Synonyms/Alias
  
Gene Name
 AP2M1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
244CKFGMNDKIVIEKQGubiquitination[1]
249NDKIVIEKQGKGTADubiquitination[1, 2, 3, 4, 5]
252IVIEKQGKGTADETSubiquitination[1, 2, 5]
263DETSKSGKQSIAIDDubiquitination[1]
281HQCVRLSKFDSERSIubiquitination[1, 3, 6]
306LMRYRTTKDIILPFRubiquitination[1, 3, 6]
333LEVKVVIKSNFKPSLubiquitination[3]
337VVIKSNFKPSLLAQKubiquitination[1, 3, 6]
370MKGKAKYKASENAIVubiquitination[3]
403ELLPTNDKKKWARPPubiquitination[2, 4, 7]
405LPTNDKKKWARPPISubiquitination[1, 5, 6, 8]
425PFAPSGLKVRYLKVFubiquitination[1, 9]
430GLKVRYLKVFEPKLNubiquitination[3]
435YLKVFEPKLNYSDHDubiquitination[1, 2, 3, 5, 6, 8]
445YSDHDVIKWVRYIGRubiquitination[1, 3, 5, 6, 8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [8] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [9] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 460 AA 
Protein Sequence
MIGGLFIYNH KGEVLISRVY RDDIGSRQAA DSAVFSSSGP FPGEWLEANR RNAVDAFRVN 60
VIHARQQVRS PVTNIARTSF FHVKRSNIWL AAVTKQNVNA AMVFEFLYKM CDVMAAYFGK 120
ISEENIKNNF VLIYELLDEI LDFGYPQNSE TGALKTFITQ QGIKSQHQTK EEQSQITSQV 180
TGQIGWRREG IKYRRNELFL DVLESVNLLM SPQGQVLSAH VSGRVVMKSY LSGMPECKFG 240
MNDKIVIEKQ GKGTADETSK SGKQSIAIDD CTFHQCVRLS KFDSERSISF IPPDGEFELM 300
RYRTTKDIIL PFRVIPLVRE VGRTKLEVKV VIKSNFKPSL LAQKIEVRIP TPLNTSGVQV 360
ICMKGKAKYK ASENAIVWKI KRMAGMKESQ ISAEIELLPT NDKKKWARPP ISMNFEVPFA 420
PSGLKVRYLK VFEPKLNYSD HDVIKWVRYI GRSGIYETRC 460 
Gene Ontology
 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0030132; C:clathrin coat of coated pit; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR015629; Clathrin_AP50.
 IPR001392; Clathrin_mu.
 IPR008968; Clathrin_mu_C.
 IPR018240; Clathrin_mu_CS.
 IPR011012; Longin-like_dom. 
Pfam
 PF00928; Adap_comp_sub 
SMART
  
PROSITE
 PS00990; CLAT_ADAPTOR_M_1
 PS00991; CLAT_ADAPTOR_M_2
 PS51072; MHD 
PRINTS
 PR00314; CLATHRINADPT.