UniProt Accession

 AEEP_ECOLI; P51981; P51982; P76048; P77345 

Genbank Protein ID

 U33213; U00096; AP009048 

Genbank Nucleotide ID

 AAC74407.2; BAA14907.1 

Protein Name

 L-Ala-D/L-Glu epimerase 

Protein Synonyms/Alias

 AE epimerase; AEE 

Gene Name

 ycjG 

Gene Synonyms/Alias

 ycjH; b1325; JW1318 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
5	***MRTVKVFEEAWP	acetylation	[1]
80	LTREELQKILPAGAA	acetylation	[1]
151	GAKLLKVKLDNHLIS	acetylation	[1]
247	RYEMVNIKLDKTGGL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, except L-Ala-L-Arg, L-Ala-L-Lys and L-Ala-L-Pro. Is also active with L-Gly-L-Glu, L-Phe-L-Glu, and L-Ser-L-Glu, but not with L-Glu-L-Glu, L-Lys-L-Glu, L-Pro-L-Glu, L-Lys-L-Ala, or D- Ala-D-Ala. 

Sequence Annotation

 ACT_SITE 151 151 Proton acceptor; specific for (R)-
 ACT_SITE 247 247 Proton acceptor; specific for (S)-
 METAL 176 176 Magnesium (By similarity).
 METAL 202 202 Magnesium (By similarity).
 METAL 225 225 Magnesium (By similarity).
 BINDING 124 124 Substrate (By similarity).
 BINDING 149 149 Substrate (By similarity).
 BINDING 275 275 Substrate; via carbonyl oxygen (By
 BINDING 297 297 Substrate (By similarity).
 BINDING 299 299 Substrate (By similarity).  

Keyword

 3D-structure; Cell wall biogenesis/degradation; Complete proteome; Isomerase; Magnesium; Metal-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 321 AA 

Protein Sequence

Gene Ontology

 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016855; F:racemase and epimerase activity, acting on amino acids and derivatives; IDA:EcoCyc.
 GO:0009063; P:cellular amino acid catabolic process; IEA:InterPro.
 GO:0009254; P:peptidoglycan turnover; IEA:UniProtKB-UniPathway. 

Interpro

 IPR026911; AE_epimerase.
 IPR018110; Mandel_Rmase/mucon_lact_enz_CS.
 IPR013342; Mandelate_racemase_C.
 IPR013341; Mandelate_racemase_N.
 IPR001354; MR_MLE. 

Pfam

 PF01188; MR_MLE
 PF02746; MR_MLE_N 

SMART

 SM00922; MR_MLE 

PROSITE

 PS00908; MR_MLE_1
 PS00909; MR_MLE_2 

PRINTS