UniProt Accession

 B3KSB0_HUMAN; B3KSB0 

Genbank Protein ID

 AC011322; AK093216; CH471191; CH471191 

Genbank Nucleotide ID

 BAG52672.1; EAW53620.1; EAW53622.1 

Protein Name

 Nuclear cap binding protein subunit 2, 20kDa, isoform CRA_a 

Protein Synonyms/Alias

 Nuclear cap-binding protein subunit 2; cDNA FLJ35897 fis, clone TESTI2009502, highly similar to Nuclear cap-binding protein subunit 2 

Gene Name

 NCBP2 

Gene Synonyms/Alias

 hCG_40296 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
50	TDWDAGFKEGRQYGR	ubiquitination	[1, 2, 3, 4, 5]
81	AGRGGYGKLAQNQ**	ubiquitination	[3, 4, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 86 AA 

Protein Sequence

Gene Ontology

 GO:0005846; C:nuclear cap binding complex; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0000339; F:RNA cap binding; IEA:InterPro.
 GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro. 

Interpro

 IPR027157; NCBP2.
 IPR012677; Nucleotide-bd_a/b_plait. 

Pfam

  

SMART

  

PROSITE

  

PRINTS