CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033123
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Eukaryotic translation initiation factor 4 gamma 1 
Protein Synonyms/Alias
  
Gene Name
 EIF4G1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
668LKVPTTEKPTVTVNFubiquitination[1, 2]
699DDDEVFEKKQKEMDEubiquitination[2]
736RRSLGNIKFIGELFKubiquitination[1, 3, 4, 5, 6]
743KFIGELFKLKMLTEAubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 757 AA 
Protein Sequence
MNQPPQIAPK RERKTIRIRD PNQGGKDITE EIMSGARTAS TPTPPQTGGG LEPQANGETP 60
QVAVIVRPDD RSQGAIIADR PGLPGPEHSP SESQPSSPSP TPSPSPVLEP GSEPNLAVLS 120
IPGDTMTTIQ MSVEESTPIS RETGEPYRLS PEPTPLAEPI LEVEVTLSKP VPESEFSSSP 180
LQAPTPLASH TVEIHEPNGM VPSEDLEPEV ESSPELAPPP ACPSESPVPI APTAQPEELL 240
NGAPSPPAVD LSPVSEPEEQ AKEVTASMAP PTIPSATPAT APSATSPAQE EEMEEEEEEE 300
EGEAGEAGEA ESEKGGEELL PPESTPIPAN LSQNLEAAAA TQVAVSVPKR RRKIKELNKK 360
EAVGDLLDAF KEANPAVPEV ENQPPAGSNP GPESEGSGVP PRPEEADETW DSKEDKIHNA 420
ENIQPGEQKY EYKSDQWKPL NLEEKKRYDR EFLLGFQFIF ASMQKPEGLP HISDVVLDKA 480
NKTPLRPLDP TRLQGINCGP DFTPSFANLG RTTLSTRGPP RGGPGGELPR GPAGLGPRRS 540
QQGPRKEPRK IIATVLMTED IKLNKAEKAW KPSSKRTAAD KDRGEEDADG SKTQDLFRRV 600
RSILNKLTPQ MFQQLMKQVT QLAIDTEERL KGVIDLIFEK AISEPNFSVA YANMCRCLMA 660
LKVPTTEKPT VTVNFRKLLL NRCQKEFEKD KDDDEVFEKK QKEMDEAATA EERGRLKEEL 720
EEARDIARRR SLGNIKFIGE LFKLKMLTEA IMHDCVV 757 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0016070; P:RNA metabolic process; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR003890; MIF4G-like_typ-3. 
Pfam
 PF02854; MIF4G 
SMART
 SM00543; MIF4G 
PROSITE
  
PRINTS