UniProt Accession

 BIN2_HUMAN; Q9UBW5; Q86VV0; Q9NWK4; Q9UKN4 

Genbank Protein ID

 AF146531; AB032698; AB032710; AK000783; AC046135; BC047686 

Genbank Nucleotide ID

 AAD54227.1; BAA88108.1; BAA88125.1; BAA91376.1; AAH47686.1 

Protein Name

 Bridging integrator 2 

Protein Synonyms/Alias

 Breast cancer-associated protein 1 

Gene Name

 BIN2 

Gene Synonyms/Alias

 BRAP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MAEGKAGGAAGL	ubiquitination	[1]
19	LFAKQVQKKFSRAQE	ubiquitination	[1]
65	HKLYKDLKNFLSAVK	ubiquitination	[2, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis. 

Sequence Annotation

 DOMAIN 28 244 BAR.
 MOD_RES 273 273 Phosphoserine.
 MOD_RES 357 357 Phosphoserine.
 MOD_RES 436 436 Phosphoserine.
 MOD_RES 438 438 Phosphothreonine.
 MOD_RES 444 444 Phosphoserine.
 MOD_RES 447 447 Phosphoserine.
 MOD_RES 458 458 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Cell junction; Cell membrane; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Membrane; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 565 AA 

Protein Sequence

Gene Ontology

 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0042995; C:cell projection; IEA:UniProtKB-KW.
 GO:0001891; C:phagocytic cup; IEA:UniProtKB-SubCell.
 GO:0002102; C:podosome; IDA:UniProtKB.
 GO:0005543; F:phospholipid binding; IDA:UniProtKB.
 GO:0060326; P:cell chemotaxis; IMP:UniProtKB.
 GO:0097320; P:membrane tubulation; IDA:UniProtKB.
 GO:0006911; P:phagocytosis, engulfment; IMP:UniProtKB.
 GO:0071800; P:podosome assembly; IMP:UniProtKB. 

Interpro

 IPR027267; AH/BAR-dom.
 IPR003005; Amphiphysin.
 IPR004148; BAR_dom. 

Pfam

 PF03114; BAR 

SMART

 SM00721; BAR 

PROSITE

 PS51021; BAR 

PRINTS

 PR01251; AMPHIPHYSIN.