CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042474
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 CDK5 regulatory subunit-associated protein 1 
Protein Synonyms/Alias
  
Gene Name
 CDK5RAP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8XCMAERLKEEILNREubiquitination[1, 2, 3, 4]
16EEILNREKMVDILAGubiquitination[1, 2]
142TRAYHRLKDDVPEEVubiquitination[1, 2, 3, 4]
150DDVPEEVKLRRLEELubiquitination[1, 2, 3, 4]
186VLVEGLSKRSATDLCubiquitination[2]
201GRNDGNLKVIFPDAEacetylation[5]
201GRNDGNLKVIFPDAEubiquitination[1, 2, 3, 4, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 256 AA 
Protein Sequence
XCMAERLKEE ILNREKMVDI LAGPDAYRDL PRLLAVAESG QQAANVLLSL DETYADVMPV 60
QTSASATSAF VYSREAYVEL VHHIRESIPG VSLSSDFIAG FCGETEEDHV QTVSLLREVQ 120
YNMGFLFAYS MRQKTRAYHR LKDDVPEEVK LRRLEELITI FREEATKANQ TSVGCTQLVL 180
VEGLSKRSAT DLCGRNDGNL KVIFPDAEME DVNNPGLRVR AQPGDYVLVK ITSASSQTLR 240
GHVLCRTTLR DSSAYC 256 
Gene Ontology
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0009451; P:RNA modification; IEA:InterPro. 
Interpro
 IPR006638; Elp3/MiaB/NifB.
 IPR023970; MeThioTfrase/rSAM.
 IPR013848; Methylthiotransferase_N.
 IPR023404; rSAM_horseshoe.
 IPR002792; TRAM_dom. 
Pfam
 PF01938; TRAM 
SMART
 SM00729; Elp3 
PROSITE
 PS51449; MTTASE_N
 PS50926; TRAM 
PRINTS