CPLM-000996

Genbank Nucleotide ID

Dolichol-phosphate mannosyltransferase

Protein Synonyms/Alias

Dolichol-phosphate mannose synthase; DPM synthase; Dolichyl-phosphate beta-D-mannosyltransferase; Mannose-P-dolichol synthase; MPD synthase

Homo sapiens (Human)

Position	Peptide	Type	References
26	VRSPRQNKYSVLLPT	ubiquitination	[1, 2, 3, 4]
80	DVAEQLEKIYGSDRI	ubiquitination	[1, 2, 3, 4, 5, 6]
95	LLRPREKKLGLGTAY	ubiquitination	[1, 2, 3, 4]
136	PEFIRKQKEGNFDIV	ubiquitination	[2, 6]
149	IVSGTRYKGNGGVYG	ubiquitination	[1]
160	GVYGWDLKRKIISRG	ubiquitination	[2, 5]
197	YRKEVLEKLIEKCVS	acetylation	[7]
240	DRVYGESKLGGNEIV	ubiquitination	[1, 2, 6]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O- mannosylation of proteins.

MOD_RES 2 2 N-acetylalanine.
MOD_RES 9 9 Phosphoserine.

Acetylation; Complete proteome; Congenital disorder of glycosylation; Disease mutation; Endoplasmic reticulum; Glycosyltransferase; Phosphoprotein; Reference proteome; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0033185; C:dolichol-phosphate-mannose synthase complex; IDA:UniProtKB.
GO:0005789; C:endoplasmic reticulum membrane; IDA:HGNC.
GO:0043178; F:alcohol binding; IEA:Compara.
GO:0004582; F:dolichyl-phosphate beta-D-mannosyltransferase activity; IDA:UniProtKB.
GO:0004169; F:dolichyl-phosphate-mannose-protein mannosyltransferase activity; IDA:HGNC.
GO:0005537; F:mannose binding; IEA:Compara.
GO:0006501; P:C-terminal protein lipidation; TAS:Reactome.
GO:0019348; P:dolichol metabolic process; IDA:MGI.
GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; TAS:Reactome.
GO:0019673; P:GDP-mannose metabolic process; IEA:Compara.
GO:0006506; P:GPI anchor biosynthetic process; IDA:UniProtKB.
GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome.
GO:0035269; P:protein O-linked mannosylation; IDA:HGNC.

IPR001173; Glyco_trans_2.

PF00535; Glycos_transf_2