CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020407
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Crooked neck-like protein 1 
Protein Synonyms/Alias
 Crooked neck homolog; hCrn 
Gene Name
 CRNKL1 
Gene Synonyms/Alias
 CRN; CGI-201; MSTP021 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
256AQWEESLKEIQRARSubiquitination[1]
381PDVKNWIKYARFEEKacetylation[2]
388KYARFEEKHAYFAHAubiquitination[1]
453QDAQELFKNYTIFEKubiquitination[3]
461NYTIFEKKFGDRRGIubiquitination[1]
475IEDIIVSKRRFQYEEubiquitination[1]
740QIYEEANKTMRNCEEubiquitination[1]
823LKLLAMAKLWKKQQQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Involved in pre-mRNA splicing process. 
Sequence Annotation
 REPEAT 222 254 HAT 1.
 REPEAT 256 288 HAT 2.
 REPEAT 290 322 HAT 3.
 REPEAT 324 355 HAT 4.
 REPEAT 357 388 HAT 5.
 REPEAT 390 425 HAT 6.
 REPEAT 427 461 HAT 7.
 REPEAT 471 503 HAT 8.
 REPEAT 505 539 HAT 9.
 REPEAT 549 585 HAT 10.
 REPEAT 587 618 HAT 11.
 REPEAT 620 652 HAT 12.
 REPEAT 654 688 HAT 13.
 REPEAT 690 721 HAT 14.
 REPEAT 726 767 HAT 15.
 REPEAT 769 807 HAT 16.
 REPEAT 809 834 HAT 17.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Polymorphism; Reference proteome; Repeat; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 848 AA 
Protein Sequence
MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ FRRLTWGCAL 60
DALHRSPCVA ASQHGVTHLI RSSRTPHSTR CRKEDAQPGH HGNGAASVTA QARGQRSVLQ 120
VPLPVPRSCL FSESFVVSVS SQSRFLASVP GTGVQRSTAA DMAASTAAGK QRIPKVAKVK 180
NKAPAEVQIT AEQLLREAKE RELELLPPPP QQKITDEEEL NDYKLRKRKT FEDNIRKNRT 240
VISNWIKYAQ WEESLKEIQR ARSIYERALD VDYRNITLWL KYAEMEMKNR QVNHARNIWD 300
RAITTLPRVN QFWYKYTYME EMLGNVAGAR QVFERWMEWQ PEEQAWHSYI NFELRYKEVD 360
RARTIYERFV LVHPDVKNWI KYARFEEKHA YFAHARKVYE RAVEFFGDEH MDEHLYVAFA 420
KFEENQKEFE RVRVIYKYAL DRISKQDAQE LFKNYTIFEK KFGDRRGIED IIVSKRRFQY 480
EEEVKANPHN YDAWFDYLRL VESDAEAEAV REVYERAIAN VPPIQEKRHW KRYIYLWINY 540
ALYEELEAKD PERTRQVYQA SLELIPHKKF TFAKMWILYA QFEIRQKNLS LARRALGTSI 600
GKCPKNKLFK VYIELELQLR EFDRCRKLYE KFLEFGPENC TSWIKFAELE TILGDIDRAR 660
AIYELAISQP RLDMPEVLWK SYIDFEIEQE ETERTRNLYR RLLQRTQHVK VWISFAQFEL 720
SSGKEGSLTK CRQIYEEANK TMRNCEEKEE RLMLLESWRS FEEEFGTASD KERVDKLMPE 780
KVKKRRKVQT DDGSDAGWEE YFDYIFPEDA ANQPNLKLLA MAKLWKKQQQ EKEDAEHHPD 840
EDVDESES 848 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IDA:UniProtKB.
 GO:0000245; P:spliceosomal complex assembly; IDA:UniProtKB. 
Interpro
 IPR003107; HAT.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical. 
Pfam
 PF02184; HAT 
SMART
 SM00386; HAT 
PROSITE
  
PRINTS