CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000362
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Synaptojanin-2 
Protein Synonyms/Alias
 Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2 
Gene Name
 SYNJ2 
Gene Synonyms/Alias
 KIAA0348 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MALSKGLRLLGRubiquitination[1]
112YPLQEEAKEEERLIAubiquitination[2]
369EDFDVFTKGENVSPRubiquitination[1]
477SNFFDGVKQEAIKLLubiquitination[1]
543TWNVNGGKQFRSNVLubiquitination[1]
1432LNNTWLSKSSDPLDSubiquitination[3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis. 
Sequence Annotation
 DOMAIN 120 444 SAC.
 DOMAIN 889 968 RRM.
 REGION 450 ? Catalytic (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Hydrolase; Membrane; Polymorphism; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1496 AA 
Protein Sequence
MALSKGLRLL GRLGAEGDCS VLLEARGRDD CLLFEAGTVA TLAPEEKEVI KGQYGKLTDA 60
YGCLGELRLK SGGTSLSFLV LVTGCTSVGR IPDAEIYKIT ATDFYPLQEE AKEEERLIAL 120
KKILSSGVFY FSWPNDGSRF DLTVRTQKQG DDSSEWGNSF FWNQLLHVPL RQHQVSCCDW 180
LLKIICGVVT IRTVYASHKQ AKACLVSRVS CERTGTRFHT RGVNDDGHVS NFVETEQMIY 240
MDDGVSSFVQ IRGSVPLFWE QPGLQVGSHH LRLHRGLEAN APAFDRHMVL LKEQYGQQVV 300
VNLLGSRGGE EVLNRAFKKL LWASCHAGDT PMINFDFHQF AKGGKLEKLE TLLRPQLKLH 360
WEDFDVFTKG ENVSPRFQKG TLRMNCLDCL DRTNTVQSFI ALEVLHLQLK TLGLSSKPIV 420
DRFVESFKAM WSLNGHSLSK VFTGSRALEG KAKVGKLKDG ARSMSRTIQS NFFDGVKQEA 480
IKLLLVGDVY GEEVADKGGM LLDSTALLVT PRILKAMTER QSEFTNFKRI RIAMGTWNVN 540
GGKQFRSNVL RTAELTDWLL DSPQLSGATD SQDDSSPADI FAVGFEEMVE LSAGNIVNAS 600
TTNKKMWGEQ LQKAISRSHR YILLTSAQLV GVCLYIFVRP YHVPFIRDVA IDTVKTGMGG 660
KAGNKGAVGI RFQFHSTSFC FICSHLTAGQ SQVKERNEDY KEITQKLCFP MGRNVFSHDY 720
VFWCGDFNYR IDLTYEEVFY FVKRQDWKKL LEFDQLQLQK SSGKIFKDFH EGAINFGPTY 780
KYDVGSAAYD TSDKCRTPAW TDRVLWWRKK HPFDKTAGEL NLLDSDLDVD TKVRHTWSPG 840
ALQYYGRAEL QASDHRPVLA IVEVEVQEVD VGARERVFQE VSSFQGPLDA TVVVNLQSPT 900
LEEKNEFPED LRTELMQTLG SYGTIVLVRI NQGQMLVTFA DSHSALSVLD VDGMKVKGRA 960
VKIRPKTKDW LKGLREEIIR KRDSMAPVSP TANSCLLEEN FDFTSLDYES EGDILEDDED 1020
YLVDEFNQPG VSDSELGGDD LSDVPGPTAL APPSKSPALT KKKQHPTYKD DADLVELKRE 1080
LEAVGEFRHR SPSRSLSVPN RPRPPQPPQR PPPPTGLMVK KSASDASISS GTHGQYSILQ 1140
TARLLPGAPQ QPPKARTGIS KPYNVKQIKT TNAQEAEAAI RCLLEARGGA SEEALSAVAP 1200
RDLEASSEPE PTPGAAKPET PQAPPLLPRR PPPRVPAIKK PTLRRTGKPL SPEEQFEQQT 1260
VHFTIGPPET SVEAPPVVTA PRVPPVPKPR TFQPGKAAER PSHRKPASDE APPGAGASVP 1320
PPLEAPPLVP KVPPRRKKSA PAAFHLQVLQ SNSQLLQGLT YNSSDSPSGH PPAAGTVFPQ 1380
GDFLSTSSAT SPDSDGTKAM KPEAAPLLGD YQDPFWNLLH HPKLLNNTWL SKSSDPLDSG 1440
TRSPKRDPID PVSAGASAAK AELPPDHEHK TLGHWVTISD QEKRTALQVF DPLAKT 1496 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0004439; F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity; IEA:EC.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
 GO:0046856; P:phosphatidylinositol dephosphorylation; IEA:InterPro.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR015047; DUF1866.
 IPR005135; Endo/exonuclease/phosphatase.
 IPR000300; IPPc.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR002013; Syja_N. 
Pfam
 PF08952; DUF1866
 PF03372; Exo_endo_phos
 PF02383; Syja_N 
SMART
 SM00128; IPPc 
PROSITE
 PS50102; RRM
 PS50275; SAC 
PRINTS