CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021094
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cysteine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Cysteinyl-tRNA synthetase; CysRS 
Gene Name
 Cars 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
122YNSLTRNKDVFIPQDubiquitination[1]
501LKNFITIKDALKKHSubiquitination[1]
575AEEVELNKNFYGKKTubiquitination[1]
797PTHDTEGKELSKGQAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
 MOTIF 140 150 "HIGH" region.
 MOTIF 489 493 "KMSKS" region.
 METAL 138 138 Zinc (By similarity).
 METAL 431 431 Zinc (By similarity).
 METAL 456 456 Zinc (By similarity).
 METAL 460 460 Zinc (By similarity).
 BINDING 492 492 ATP (By similarity).
 MOD_RES 390 390 Phosphoserine.
 MOD_RES 829 829 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Metal-binding; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 831 AA 
Protein Sequence
MAGSSAEQAA DYRSILSISD EAARVQALDQ HLSTRSYIQG YSLSQADVDV FRQLSAPPAD 60
SRLFHVARWF RHIEALLGGP QGRDEPCRLQ ASKGRRVQPQ WSPPAGTEPC RLRLYNSLTR 120
NKDVFIPQDG KKVTWYCCGP TVYDASHMGH ARSYISFDIL RRVLRDYFQY DVFYCMNITD 180
IDDKIIRRAR QNYLFEQYRE QKPPATQLLK DVRDAMKPFS VKLSETTDPD KRQMLERIQN 240
SVKLATEPLE QAVRSSLSGE EVDSKVQVLL EEAKDLLSDW LDSTGGSEVT DNSIFSKLPK 300
FWEEEFHKDM EALNVLPPDV LTRVSEYVPE IVNFVQKIVD NGYGYASNGS VYFDTAKFAA 360
SEKHSYGKLV PEAVGDQKAL QEGEGDLSIS ADRLSEKRSP NDFALWKASK PGEPSWPCPW 420
GKGRPGWHIE CSAMAGTLLG ASMDIHGGGF DLRFPHHDNE LAQSEAYFEN DCWVRYFLHT 480
GHLTIAGCKM SKSLKNFITI KDALKKHSAR QLRLAFLMHS WKDTLDYSSN TMESALQYEK 540
FMNEFFLNVK DILRAPVDIT GQFEKWEAEE VELNKNFYGK KTAVHEALCD NIDTRTVMEE 600
MRALVSQCNL YMAARKAERR RPNRALLENI AMYLTHMLKI FGAIEEESPL GFPVGGPGTN 660
LNLESTVMPY LQVLSEFREG VRKIAREKKV LEVLQLSDAL RDDILPELGV RFEDHEGLPT 720
VVKLVDRDTL LKEKEGKKRA EEEKRRKKEE AARKKQEQEA AKLAKMKIPP SEMFLSEVNK 780
YSKFDENGLP THDTEGKELS KGQAKKLKKL FEAQEKLYKE YLQMLQNGSL Q 831 
Gene Ontology
 GO:0005829; C:cytosol; IBA:RefGenome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004817; F:cysteine-tRNA ligase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000049; F:tRNA binding; ISS:UniProtKB.
 GO:0006423; P:cysteinyl-tRNA aminoacylation; ISS:UniProtKB. 
Interpro
 IPR015803; Cys-tRNA-ligase.
 IPR024909; Cys-tRNA/MSH_ligase.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 
Pfam
 PF01406; tRNA-synt_1e 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00983; TRNASYNTHCYS.