CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011970
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cleavage stimulation factor subunit 3 
Protein Synonyms/Alias
 CF-1 77 kDa subunit; Cleavage stimulation factor 77 kDa subunit; CSTF 77 kDa subunit; CstF-77 
Gene Name
 CSTF3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17AAEYVPEKVKKAEKKubiquitination[1]
20YVPEKVKKAEKKLEEubiquitination[1]
49AQNQPIDKARKTYERubiquitination[1]
94LFQRCLMKVLHIDLWubiquitination[1, 2, 3, 4, 5]
192QLWRDYNKYEEGINIubiquitination[2, 5]
274EDQTLITKRVMFAYEubiquitination[6]
682ESNAVLTKAVKRPNEubiquitination[1, 4, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. 
Sequence Annotation
 REPEAT 45 77 HAT 1.
 REPEAT 79 110 HAT 2.
 REPEAT 117 152 HAT 3.
 REPEAT 163 196 HAT 4.
 REPEAT 221 261 HAT 5.
 REPEAT 271 303 HAT 6.
 REPEAT 319 352 HAT 7.
 REPEAT 354 387 HAT 8.
 REPEAT 458 494 HAT 9.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 691 691 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 717 AA 
Protein Sequence
MSGDGATEQA AEYVPEKVKK AEKKLEENPY DLDAWSILIR EAQNQPIDKA RKTYERLVAQ 60
FPSSGRFWKL YIEAEIKAKN YDKVEKLFQR CLMKVLHIDL WKCYLSYVRE TKGKLPSYKE 120
KMAQAYDFAL DKIGMEIMSY QIWVDYINFL KGVEAVGSYA ENQRITAVRR VYQRGCVNPM 180
INIEQLWRDY NKYEEGINIH LAKKMIEDRS RDYMNARRVA KEYETVMKGL DRNAPSVPPQ 240
NTPQEAQQVD MWKKYIQWEK SNPLRTEDQT LITKRVMFAY EQCLLVLGHH PDIWYEAAQY 300
LEQSSKLLAE KGDMNNAKLF SDEAANIYER AISTLLKKNM LLYFAYADYE ESRMKYEKVH 360
SIYNRLLAIE DIDPTLVYIQ YMKFARRAEG IKSGRMIFKK AREDTRTRHH VYVTAALMEY 420
YCSKDKSVAF KIFELGLKKY GDIPEYVLAY IDYLSHLNED NNTRVLFERV LTSGSLPPEK 480
SGEIWARFLA FESNIGDLAS ILKVEKRRFT AFKEEYEGKE TALLVDRYKF MDLYPCSASE 540
LKALGYKDVS RAKLAAIIPD PVVAPSIVPV LKDEVDRKPE YPKPDTQQMI PFQPRHLAPP 600
GLHPVPGGVF PVPPAAVVLM KLLPPPICFQ GPFVQVDELM EIFRRCKIPN TVEEAVRIIT 660
GGAPELAVEG NGPVESNAVL TKAVKRPNED SDEDEEKGAV VPPVHDIYRA RQQKRIR 717 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0006379; P:mRNA cleavage; TAS:ProtInc.
 GO:0006378; P:mRNA polyadenylation; TAS:ProtInc.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 
Interpro
 IPR003107; HAT.
 IPR008847; Suf. 
Pfam
 PF05843; Suf 
SMART
 SM00386; HAT 
PROSITE
  
PRINTS