CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011950
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heat shock protein 75 kDa, mitochondrial 
Protein Synonyms/Alias
 HSP 75; TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1 
Gene Name
 TRAP1 
Gene Synonyms/Alias
 HSP75 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
109ARSLYSEKEVFIRELubiquitination[1, 2]
181TIARSGSKAFLDALQubiquitination[3]
332PRYTLHYKTDAPLNIacetylation[4]
382KATDILPKWLRFIRGacetylation[4]
382KATDILPKWLRFIRGubiquitination[5]
424VLQQRLIKFFIDQSKacetylation[4]
431KFFIDQSKKDAEKYAacetylation[4]
466EVKEDIAKLLRYESSacetylation[4]
652NPRHALIKKLNQLRAacetylation[6]
699RLNELLVKALERH**ubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330] 
Functional Description
 Chaperone that expresses an ATPase activity. 
Sequence Annotation
 BINDING 119 119 ATP (By similarity).
 BINDING 158 158 ATP (By similarity).
 BINDING 171 171 ATP (By similarity).
 BINDING 205 205 ATP; via amide nitrogen (By similarity).
 BINDING 402 402 ATP (By similarity).
 MOD_RES 87 87 N6-acetyllysine (By similarity).
 MOD_RES 170 170 Phosphoserine (By similarity).
 MOD_RES 174 174 Phosphothreonine (By similarity).
 MOD_RES 332 332 N6-acetyllysine.
 MOD_RES 401 401 Phosphoserine.
 MOD_RES 424 424 N6-acetyllysine.
 MOD_RES 466 466 N6-acetyllysine.
 MOD_RES 494 494 Phosphothreonine.  
Keyword
 Acetylation; ATP-binding; Chaperone; Complete proteome; Mitochondrion; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 704 AA 
Protein Sequence
MARELRALLL WGRRLRPLLR APALAAVPGG KPILCPRRTT AQLGPRRNPA WSLQAGRLFS 60
TQTAEDKEEP LHSIISSTES VQGSTSKHEF QAETKKLLDI VARSLYSEKE VFIRELISNA 120
SDALEKLRHK LVSDGQALPE MEIHLQTNAE KGTITIQDTG IGMTQEELVS NLGTIARSGS 180
KAFLDALQNQ AEASSKIIGQ FGVGFYSAFM VADRVEVYSR SAAPGSLGYQ WLSDGSGVFE 240
IAEASGVRTG TKIIIHLKSD CKEFSSEARV RDVVTKYSNF VSFPLYLNGR RMNTLQAIWM 300
MDPKDVREWQ HEEFYRYVAQ AHDKPRYTLH YKTDAPLNIR SIFYVPDMKP SMFDVSRELG 360
SSVALYSRKV LIQTKATDIL PKWLRFIRGV VDSEDIPLNL SRELLQESAL IRKLRDVLQQ 420
RLIKFFIDQS KKDAEKYAKF FEDYGLFMRE GIVTATEQEV KEDIAKLLRY ESSALPSGQL 480
TSLSEYASRM RAGTRNIYYL CAPNRHLAEH SPYYEAMKKK DTEVLFCFEQ FDELTLLHLR 540
EFDKKKLISV ETDIVVDHYK EEKFEDRSPA AECLSEKETE ELMAWMRNVL GSRVTNVKVT 600
LRLDTHPAMV TVLEMGAARH FLRMQQLAKT QEERAQLLQP TLEINPRHAL IKKLNQLRAS 660
EPGLAQLLVD QIYENAMIAA GLVDDPRAMV GRLNELLVKA LERH 704 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005164; F:tumor necrosis factor receptor binding; NAS:UniProtKB.
 GO:0034599; P:cellular response to oxidative stress; IDA:BHF-UCL.
 GO:0006457; P:protein folding; IEA:InterPro. 
Interpro
 IPR003594; HATPase_ATP-bd.
 IPR001404; Hsp90.
 IPR020575; Hsp90_N.
 IPR020568; Ribosomal_S5_D2-typ_fold. 
Pfam
 PF02518; HATPase_c
 PF00183; HSP90 
SMART
 SM00387; HATPase_c 
PROSITE
 PS00298; HSP90 
PRINTS
 PR00775; HEATSHOCK90.