CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042603
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Cytosol aminopeptidase 
Protein Synonyms/Alias
  
Gene Name
 LAP3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22GDQEAWQKGVLFASGubiquitination[1, 2, 3, 4, 5]
55RFAEIIEKNLKSASSacetylation[6, 7]
55RFAEIIEKNLKSASSubiquitination[1, 4, 5]
58EIIEKNLKSASSKTEubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 209 AA 
Protein Sequence
QKKKMAVSAK LYGSGDQEAW QKGVLFASGQ NLARQLMETP ANEMTPTRFA EIIEKNLKSA 60
SSKTEVHIRP KSWIEEQAMG SFLSVAKGSD EPPVFLEIHY KGSPNANEPP LVFVGKGITF 120
DSGGISIKAS ANMDLMRADM GGAATICSAI VSAAKLNLPI NIIGAMDVAL GSGATGVFTN 180
SSWLWNKLFE ASIETGDRVW RMPLFEHYT 209 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0004177; F:aminopeptidase activity; IEA:InterPro.
 GO:0030145; F:manganese ion binding; IEA:InterPro.
 GO:0008235; F:metalloexopeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:InterPro. 
Interpro
 IPR011356; Leucine_aapep/pepB.
 IPR000819; Peptidase_M17_C. 
Pfam
 PF00883; Peptidase_M17 
SMART
  
PROSITE
  
PRINTS
 PR00481; LAMNOPPTDASE.