UniProt Accession

 BUB3_MOUSE; Q9WVA3; P97397; Q6ZWM5 

Genbank Protein ID

 AF149822; U67327; AK088534; BC025089 

Genbank Nucleotide ID

 AAD38038.1; AAB39606.1; BAC40409.1; AAH25089.1 

Protein Name

 Mitotic checkpoint protein BUB3 

Protein Synonyms/Alias

 WD repeat type I transmembrane protein A72.5 

Gene Name

 Bub3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
216	DPSPEVQKKKYAFKC	acetylation	[1]
216	DPSPEVQKKKYAFKC	ubiquitination	[2]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1 (By similarity). Regulates chromosome segregation during oocyte meiosis. 

Sequence Annotation

 REPEAT 5 43 WD 1.
 REPEAT 46 83 WD 2.
 REPEAT 86 124 WD 3.
 REPEAT 128 163 WD 4.
 REPEAT 223 262 WD 5.
 MOD_RES 179 179 N6-acetyllysine (By similarity).
 MOD_RES 211 211 Phosphoserine (By similarity).
 CROSSLNK 216 216 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; ADP-ribosylation; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Complete proteome; Isopeptide bond; Kinetochore; Meiosis; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 326 AA 

Protein Sequence

Gene Ontology

 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008608; P:attachment of spindle microtubules to kinetochore; IEA:Compara.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0000070; P:mitotic sister chromatid segregation; IMP:MGI.
 GO:0051983; P:regulation of chromosome segregation; IDA:UniProtKB.
 GO:0071173; P:spindle assembly checkpoint; IDA:UniProtKB. 

Interpro

 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS

 PR00320; GPROTEINBRPT.