UniProt Accession

 KCRS_MOUSE; Q6P8J7 

Genbank Protein ID

 BC061221 

Genbank Nucleotide ID

 AAH61221.1 

Protein Name

 Creatine kinase S-type, mitochondrial 

Protein Synonyms/Alias

 Basic-type mitochondrial creatine kinase; Mib-CK; Sarcomeric mitochondrial creatine kinase; S-MtCK 

Gene Name

 Ckmt2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
59	ADYPDLRKHNNCMAE	ubiquitination	[1]
75	LTPTIYAKLRNKMTP	ubiquitination	[1]
140	GYDPRVMKHPTDLDA	ubiquitination	[1]
149	PTDLDASKITHGQFD	ubiquitination	[1]
209	DLAGRYYKLSEMTEQ	ubiquitination	[1]
344	SKDPRFSKILENLRL	acetylation	[2]
344	SKDPRFSKILENLRL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 

Functional Description

 Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (By similarity). 

Sequence Annotation

 DOMAIN 46 132 Phosphagen kinase N-terminal.
 DOMAIN 159 401 Phosphagen kinase C-terminal.
 NP_BIND 162 166 ATP (By similarity).
 NP_BIND 354 359 ATP (By similarity).
 REGION 40 64 Cardiolipin-binding (By similarity).
 BINDING 225 225 ATP (By similarity).
 BINDING 270 270 ATP (By similarity).
 BINDING 326 326 ATP (By similarity).
 BINDING 369 369 ATP (By similarity).  

Keyword

 ATP-binding; Complete proteome; Direct protein sequencing; Kinase; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Reference proteome; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 419 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004111; F:creatine kinase activity; TAS:MGI.
 GO:0006603; P:phosphocreatine metabolic process; TAS:MGI. 

Interpro

 IPR000749; ATP-guanido_PTrfase.
 IPR022415; ATP-guanido_PTrfase_AS.
 IPR022414; ATP-guanido_PTrfase_cat.
 IPR022413; ATP-guanido_PTrfase_N.
 IPR014746; Gln_synth/guanido_kin_cat_dom. 

Pfam

 PF00217; ATP-gua_Ptrans
 PF02807; ATP-gua_PtransN 

SMART

  

PROSITE

 PS00112; PHOSPHAGEN_KINASE
 PS51510; PHOSPHAGEN_KINASE_C
 PS51509; PHOSPHAGEN_KINASE_N 

PRINTS