UniProt Accession

 CENPH_HUMAN; Q9H3R5; A8K3Y1 

Genbank Protein ID

 AB035124; AK290746; CH471137; BC015355; BC012024 

Genbank Nucleotide ID

 BAB18635.1; BAF83435.1; EAW51304.1; AAH15355.1; AAH12024.1 

Protein Name

 Centromere protein H 

Protein Synonyms/Alias

 CENP-H; Interphase centromere complex protein 35 

Gene Name

 CENPH 

Gene Synonyms/Alias

 ICEN35 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
51	LRLRAQTKQQLLEYK	ubiquitination	[1, 2]
76	PEQIMQEKQIEAKIE	ubiquitination	[2, 3, 4, 5]
81	QEKQIEAKIEDLENE	ubiquitination	[2]
118	ALKKNLEKISRQSSV	ubiquitination	[2]
131	SVLMDNMKHLLELNK	ubiquitination	[2, 3, 5]
138	KHLLELNKLIMKSQQ	ubiquitination	[2]
142	ELNKLIMKSQQESWD	ubiquitination	[2, 4]
165	RKKRLQLKQASESKL	ubiquitination	[2]
171	LKQASESKLLEIQTE	ubiquitination	[2]
179	LLEIQTEKNKQKIDL	ubiquitination	[2, 6]
181	EIQTEKNKQKIDLDS	ubiquitination	[2]
183	QTEKNKQKIDLDSME	ubiquitination	[2]
234	WAEDPALKEIVLQLE	ubiquitination	[2]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine.  

Keyword

 Acetylation; Centromere; Chromosome; Coiled coil; Complete proteome; Kinetochore; Nucleus; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 247 AA 

Protein Sequence

Gene Ontology

 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000776; C:kinetochore; IMP:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0043515; F:kinetochore binding; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:InterPro.
 GO:0034080; P:CENP-A containing nucleosome assembly at centromere; TAS:Reactome.
 GO:0007059; P:chromosome segregation; IEA:InterPro.
 GO:0051383; P:kinetochore organization; TAS:UniProtKB.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 

Interpro

 IPR008426; Centromere_CenpH. 

Pfam

 PF05837; CENP-H 

SMART

  

PROSITE

  

PRINTS