CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001548
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Epsin-2 
Protein Synonyms/Alias
 EPS-15-interacting protein 2 
Gene Name
 EPN2 
Gene Synonyms/Alias
 KIAA1065 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11SSIRRQMKNIVNNYSubiquitination[1]
107IFAIQTLKDFQYIDRubiquitination[1, 2, 3]
117QYIDRDGKDQGINVRubiquitination[2]
135KQLVALLKDEERLKAubiquitination[2, 4]
149AERAQALKTKERMAQubiquitination[2]
188HSEQEYGKAGGSPASubiquitination[2, 4]
423QPASSAGKRASDAWGubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Plays a role in the formation of clathrin-coated invaginations and endocytosis. 
Sequence Annotation
 DOMAIN 12 144 ENTH.
 REPEAT 275 294 UIM 1.
 REPEAT 300 319 UIM 2.
 REPEAT 352 354 1.
 REPEAT 364 366 2.
 REPEAT 377 379 3.
 REPEAT 391 393 4.
 REPEAT 409 411 5.
 REPEAT 427 429 6.
 REPEAT 537 539 1.
 REPEAT 552 554 2.
 REPEAT 637 639 3.
 REGION 352 639 6 X 3 AA repeats of [DE]-P-W.
 REGION 537 639 3 X 3 AA repeats of N-P-F.
 BINDING 8 8 Phosphatidylinositol lipid headgroup (By
 BINDING 11 11 Phosphatidylinositol lipid headgroup (By
 BINDING 25 25 Phosphatidylinositol lipid headgroup (By
 BINDING 30 30 Phosphatidylinositol lipid headgroup (By
 BINDING 63 63 Phosphatidylinositol lipid headgroup (By
 BINDING 73 73 Phosphatidylinositol lipid headgroup (By
 MOD_RES 173 173 Phosphoserine (By similarity).
 MOD_RES 182 182 Phosphoserine (By similarity).
 MOD_RES 192 192 Phosphoserine (By similarity).
 MOD_RES 195 195 Phosphoserine (By similarity).
 MOD_RES 485 485 Phosphothreonine (By similarity).
 MOD_RES 486 486 Phosphoserine (By similarity).
 MOD_RES 492 492 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Endocytosis; Lipid-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 641 AA 
Protein Sequence
MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV VAFSEIMSMV 60
WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI FAIQTLKDFQ YIDRDGKDQG 120
INVREKSKQL VALLKDEERL KAERAQALKT KERMAQVATG MGSNQITFGR GSSQPNLSTS 180
HSEQEYGKAG GSPASYHGSP EASLCPQHRT GAPLGQSEEL QPLSQRHPFL PHLGLASRPN 240
GDWSQPCLTC DRAARATSPR VSSELEQARP QTSGEEELQL QLALAMSREV AEQEERLRRG 300
DDLRLQMALE ESRRDTVKIP KKKEHGSLPQ QTTLLDLMDA LPSSGPAAQK AEPWGPSAST 360
NQTNPWGGPA APASTSDPWP SFGTKPAASI DPWGVPTGAT VQSVPKNSDP WAASQQPASS 420
AGKRASDAWG AVSTTKPVSV SGSFELFSNL NGTIKDDFSE FDNLRTSKKT AESVTSLPSQ 480
NNGTTSPDPF ESQPLTVASS KPSSARKTPE SFLGPNAALV NLDSLVTRPA PPAQSLNPFL 540
APGAPATSAP VNPFQVNQPQ PLTLNQLRGS PVLGTSTSFG PGPGVESMAV ASMTSAAPQP 600
ALGATGSSLT PLGPAMMNMV GSVGIPPSAA QATGTTNPFL L 641 
Gene Ontology
 GO:0030128; C:clathrin coat of endocytic vesicle; IEA:InterPro.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0048568; P:embryonic organ development; IEA:Compara.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0007219; P:Notch signaling pathway; IEA:Compara. 
Interpro
 IPR008942; ENTH_VHS.
 IPR027319; Epsin-2_metazoa.
 IPR013809; Epsin-like_N.
 IPR001026; Epsin_dom_N.
 IPR003903; Ubiquitin-int_motif. 
Pfam
 PF01417; ENTH 
SMART
 SM00273; ENTH
 SM00726; UIM 
PROSITE
 PS50942; ENTH
 PS50330; UIM 
PRINTS