CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015339
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein TMED8 
Protein Synonyms/Alias
  
Gene Name
 TMED8 
Gene Synonyms/Alias
 FAM15B; L10 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
151DLLGDHRKVSPPLMAubiquitination[1]
169IWTFAKVKEFKSKLGacetylation[2]
172FAKVKEFKSKLGKEKacetylation[2]
304GEGIYLLKFDNSYSLubiquitination[1, 3, 4, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 DOMAIN 159 323 GOLD.
 MOD_RES 21 21 Phosphoserine (By similarity).
 MOD_RES 169 169 N6-acetyllysine.
 MOD_RES 172 172 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 325 AA 
Protein Sequence
MSDLQAAEGP GSWSPTARPG SAGGVGDCQG VEGSQAAASE NEDLENKDTS LLASATDPEP 60
CSSPHRPQMV SPVSKDATED LRKATGPLEA QALVKQDLLP ADQAQVLNEM AKYQVPQRSG 120
DIVMIQSEHT GAIDVLSADL ESADLLGDHR KVSPPLMAPP CIWTFAKVKE FKSKLGKEKN 180
SRLVVKRGEV VTIRVPTHPE GKRVCWEFAT DDYDIGFGVY FDWTPVTSTD ITVQVSDSSD 240
DEDEEEEEEE EIEEPVPAGD VERGSRSSLR GRYGEVMPVY RRDSHRDVQA GSHDYPGEGI 300
YLLKFDNSYS LLRNKTLYFH IYYTS 325 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0006810; P:transport; IEA:InterPro. 
Interpro
 IPR009038; GOLD. 
Pfam
 PF13897; GOLD_2 
SMART
  
PROSITE
 PS50866; GOLD 
PRINTS