CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015171
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear factor related to kappa-B-binding protein 
Protein Synonyms/Alias
 DNA-binding protein R kappa-B; INO80 complex subunit G 
Gene Name
 NFRKB 
Gene Synonyms/Alias
 INO80G 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
122NPLHIAQKLFRDGHFubiquitination[1]
135HFNPEVVKYRQLCFKubiquitination[1]
161QYFHRLLKQILASRSubiquitination[1, 2]
207RTQQRYLKVLREVKEubiquitination[1]
321TRVNAGRKGSLAALYubiquitination[1]
581PVKGVFDKETSLNKAubiquitination[1]
956ITGQLGVKPQTGNSIubiquitination[1]
975TNFRIQGKDVLRLPPubiquitination[1]
1007DMMATLAKSQVTTVKubiquitination[1]
1029TGGNTTGKGISATLHubiquitination[1]
1074VKVTPDLKPTEASSSubiquitination[1]
1223ISQPMKGKSVVTAPIubiquitination[1]
1232VVTAPIIKGNLGANLubiquitination[1]
1262TKLIAGNKPVSFLTAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Binds to the DNA consensus sequence 5'-GGGGAATCTCC-3'. 
Sequence Annotation
 REGION 370 495 Winged-helix like domain.
 MOD_RES 298 298 Phosphoserine.
 MOD_RES 1022 1022 Phosphoserine.
 MOD_RES 1237 1237 N6-acetyllysine.
 MOD_RES 1291 1291 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1299 AA 
Protein Sequence
MHVFVQSTCG EETMDSLDHM LTDPLELGPC GDGHGTRIME DCLLGGTRVS LPEDLLEDPE 60
IFFDVVSLST WQEVLSDSQR EHLQQFLPQF PEDSAEQQNE LILALFSGEN FRFGNPLHIA 120
QKLFRDGHFN PEVVKYRQLC FKSQYKRYLN SQQQYFHRLL KQILASRSDL LEMARRSGPA 180
LPFRQKRPSP SRTPEEREWR TQQRYLKVLR EVKEECGDTA LSSDEEATLD LQEQFSFEDL 240
SSWLPSSPAR SPSPAVPLRV VPTLSTTDMK TADKVELGDS DLKIMLKKHH EKRKHQPDHP 300
DLLTGDLTLN DIMTRVNAGR KGSLAALYDL AVLKKKVKEK EEKKKKKIKT IKSEAEDLAE 360
PLSSTEGVAP LSQAPSPLAI PAIKEEPLED LKPCLGINEI SSSFFSLLLE ILLLESQASL 420
PMLEERVLDW QSSPASSLNS WFSAAPNWAE LVLPALQYLA GESRAVPSSF SPFVEFKEKT 480
QQWKLLGQSQ DNEKELAALF QLWLETKDQA FCKQENEDSS DATTPVPRVR TDYVVRPSTG 540
EEKRVFQEQE RYRYSQPHKA FTFRMHGFES VVGPVKGVFD KETSLNKARE HSLLRSDRPA 600
YVTILSLVRD AAARLPNGEG TRAEICELLK DSQFLAPDVT STQVNTVVSG ALDRLHYEKD 660
PCVKYDIGRK LWIYLHRDRS EEEFERIHQA QAAAAKARKA LQQKPKPPSK VKSSSKESSI 720
KVLSSGPSEQ SQMSLSDSSM PPTPVTPVTP TTPALPAIPI SPPPVSAVNK SGPSTVSEPA 780
KSSSGVLLVS SPTMPHLGTM LSPASSQTAP SSQAAARVVS HSGSAGLSQV RVVAQPSLPA 840
VPQQSGGPAQ TLPQMPAGPQ IRVPATATQT KVVPQTVMAT VPVKAQTTAA TVQRPGPGQT 900
GLTVTSLPAT ASPVSKPATS SPGTSAPSAS TAAVIQNVTG QNIIKQVAIT GQLGVKPQTG 960
NSIPLTATNF RIQGKDVLRL PPSSITTDAK GQTVLRITPD MMATLAKSQV TTVKLTQDLF 1020
GTGGNTTGKG ISATLHVTSN PVHAADSPAK ASSASAPSST PTGTTVVKVT PDLKPTEASS 1080
SAFRLMPALG VSVADQKGKS TVASSEAKPA ATIRIVQGLG VMPPKAGQTI TVATHAKQGA 1140
SVASGSGTVH TSAVSLPSMN AAVSKTVAVA SGAASTPISI STGAPTVRQV PVSTTVVSTS 1200
QAGKLPTRIT VPLSVISQPM KGKSVVTAPI IKGNLGANLS GLGRNIILTT MPAGTKLIAG 1260
NKPVSFLTAQ QLQQLQQQGQ ATQVRIQTVP ASHLQQGTAS GSSKAVSTVV VTTAPSPKQA 1320
PEQQ 1324 
Gene Ontology
 GO:0031011; C:Ino80 complex; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0006954; P:inflammatory response; TAS:ProtInc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR024867; NFRKB.
 IPR025220; NFRKB_winged_dom. 
Pfam
 PF14465; NFRKB_winged 
SMART
  
PROSITE
  
PRINTS