CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019352
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinetochore-associated protein NSL1 homolog 
Protein Synonyms/Alias
  
Gene Name
 NSL1 
Gene Synonyms/Alias
 C1orf48; DC31; DC8; MIS14 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59LCGRFVQKLGDALPEubiquitination[1, 2]
125IIVDIATKRKQYPRKubiquitination[1]
132KRKQYPRKILECVIKubiquitination[2]
174APHMENLKCRGETVAubiquitination[2, 3]
189KEISEAMKSLPALIEubiquitination[1, 2, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. 
Sequence Annotation
 MOD_RES 244 244 Phosphothreonine.  
Keyword
 Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Complete proteome; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 281 AA 
Protein Sequence
MAGSPELVVL DPPWDKELAA GTESQALVSA TPREDFRVRC TSKRAVTEML QLCGRFVQKL 60
GDALPEEIRE PALRDAQWTF ESAVQENISI NGQAWQEASD NCFMDSDIKV LEDQFDEIIV 120
DIATKRKQYP RKILECVIKT IKAKQEILKQ YHPVVHPLDL KYDPDPAPHM ENLKCRGETV 180
AKEISEAMKS LPALIEQGEG FSQVLRMQPV IHLQRIHQEV FSSCHRKPDA KPENFITQIE 240
TTPTETASRK TSDMVLKRKQ TKDCPQRKWY PLRPKKINLD T 281 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000444; C:MIS12/MIND type complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 
Interpro
 IPR013950; Kinetochore_Mis14. 
Pfam
 PF08641; Mis14 
SMART
  
PROSITE
  
PRINTS