UniProt Accession

 NACA_YEAST; P38879; D3DLE1 

Genbank Protein ID

 U17134; U00030; AY558288; L12222; BK006934 

Genbank Nucleotide ID

 AAA92080.1; AAB68367.1; AAS56614.1; AAC15849.1; DAA06885.1 

Protein Name

 Nascent polypeptide-associated complex subunit alpha 

Protein Synonyms/Alias

 NAC-alpha; Alpha-NAC; GAL4 DNA-binding enhancer protein 2 

Gene Name

 EGD2 

Gene Synonyms/Alias

 YHR193C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
15	ANVTVLNKNEKKARE	acetylation	[1]
26	KARELIGKLGLKQIP	acetylation	[1]
30	LIGKLGLKQIPGIIR	acetylation	[1]
30	LIGKLGLKQIPGIIR	ubiquitination	[2]
52	NQIYAIEKPEVFRSA	acetylation	[1]
156	VSKNQAIKALKAHNG	acetylation	[1]
156	VSKNQAIKALKAHNG	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Component of the nascent polypeptide-associated complex (NAC), a dynamic component of the ribosomal exit tunnel, protecting the emerging polypeptides from interaction with other cytoplasmic proteins to ensure appropriate nascent protein targeting. The NAC complex also promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane and blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum. EGD2 may also be involved in transcription regulation. 

Sequence Annotation

 DOMAIN 15 73 NAC-A/B.
 DOMAIN 135 174 UBA.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 93 93 Phosphoserine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 174 AA 

Protein Sequence

Gene Ontology

 GO:0005854; C:nascent polypeptide-associated complex; IDA:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0070300; F:phosphatidic acid binding; IDA:SGD.
 GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IDA:SGD.
 GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD.
 GO:0070273; F:phosphatidylinositol-4-phosphate binding; IDA:SGD.
 GO:0051082; F:unfolded protein binding; IMP:SGD.
 GO:0051083; P:'de novo' cotranslational protein folding; IPI:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR016641; EGD2/NACA.
 IPR002715; Nas_poly-pep-assoc_cplx_dom.
 IPR000449; UBA/transl_elong_EF1B_N. 

Pfam

 PF01849; NAC
 PF00627; UBA 

SMART

  

PROSITE

 PS51151; NAC_AB
 PS50030; UBA 

PRINTS