CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014345
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin/ISG15 ligase TRIM25 
Protein Synonyms/Alias
 Estrogen-responsive finger protein; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147 
Gene Name
 Trim25 
Gene Synonyms/Alias
 Efp; Zfp147; Znf147 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
200ASADLEYKLRNKLTIubiquitination[1]
225ALEDVRSKQQCVQDSubiquitination[1]
234QCVQDSMKRKMEQLRubiquitination[1]
282TIYQVLVKKKSEMQKubiquitination[1]
309DEFEFLEKAAKLQGEubiquitination[1]
312EFLEKAAKLQGESTKubiquitination[1]
319KLQGESTKPVYIPKIubiquitination[1]
376DQTQSTFKPVQPSKKubiquitination[1]
382FKPVQPSKKTIQEKKubiquitination[1]
383KPVQPSKKTIQEKKTacetylation[2]
388SKKTIQEKKTKKTPVacetylation[2]
407PSHFSPNKLPTFGAPubiquitination[1]
421PGQSLDSKATSPDAAubiquitination[1]
430TSPDAAPKASAAQPDubiquitination[1]
442QPDSVGVKAKVLENFubiquitination[1]
444DSVGVKAKVLENFLTubiquitination[1]
480NKVSLSNKYTTASVSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs (By similarity). 
Sequence Annotation
 DOMAIN 444 634 B30.2/SPRY.
 ZN_FING 13 54 RING-type.
 MOD_RES 99 99 Phosphoserine (By similarity).
 MOD_RES 272 272 N6-acetyllysine (By similarity).
 MOD_RES 572 572 N6-acetyllysine (By similarity).
 CROSSLNK 116 116 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Antiviral defense; Coiled coil; Complete proteome; Cytoplasm; Immunity; Innate immunity; Isopeptide bond; Ligase; Metal-binding; Phosphoprotein; Reference proteome; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 634 AA 
Protein Sequence
MAELNPLAEE LSCSVCLELF KEPVTTPCGH NFCMSCLDET WVVQGPPYRC PQCRKVYQVR 60
PQLQKNTVMC AVVEQFLQAE QARTPVDDWT PPARFSASSA ATQVACDHCL TEIAVKTCLV 120
CMASFCQEHL RPHFDSPAFQ DHPLQSPIRD LLRRKCTQHN RLRELFCPEH GECICHICLV 180
EHKTCSPTTL SQASADLEYK LRNKLTIMHS HINGATKALE DVRSKQQCVQ DSMKRKMEQL 240
RQEYMEMKAV IDAAETSSLR KLKEEEKRVY GKFDTIYQVL VKKKSEMQKL KAEVELIMDK 300
GDEFEFLEKA AKLQGESTKP VYIPKIDLDH DLIMGIYQGA ADLKSELKHS IKKLQKKSEE 360
HNGSGNKGDQ TQSTFKPVQP SKKTIQEKKT KKTPVAPGPP SHFSPNKLPT FGAPGQSLDS 420
KATSPDAAPK ASAAQPDSVG VKAKVLENFL TKSRTELLEY FVKVIFDYNT AHNKVSLSNK 480
YTTASVSDGL QHYRSHPQRF TYCSQVLGLH CYKNGIHYWE VELQKNNFCG VGICYGSMER 540
QGPESRLGRN PNSWCVEWFN NKISAWHNNV EKTLPSTKAT RVGVLLNCDH GFVIFFAVTE 600
KVHLMYKFKV DFTEALYPAF WVFSAGTTLS ICSK 634 
Gene Ontology
 GO:0030054; C:cell junction; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:EC.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR003879; Butyrophylin.
 IPR008985; ConA-like_lec_gl_sf.
 IPR006574; PRY.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF00622; SPRY 
SMART
 SM00589; PRY
 SM00184; RING
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS
 PR01407; BUTYPHLNCDUF.