CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022823
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol 4-kinase beta 
Protein Synonyms/Alias
 PI4K-beta; PI4Kbeta; PtdIns 4-kinase beta; NPIK; PI4K92 
Gene Name
 PI4KB 
Gene Synonyms/Alias
 PIK4CB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
58ACQEVLEKVKLLHGGubiquitination[1]
337KSLMAIGKRLATLPTubiquitination[1]
345RLATLPTKEQKTQRLubiquitination[1]
348TLPTKEQKTQRLISEubiquitination[1]
362ELSLLNHKLPARVWLubiquitination[1]
596ERVPLWIKPYKILVIubiquitination[1]
623AVSIHQVKKQSQLSLubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity). 
Sequence Annotation
 DOMAIN 29 242 PIK helical.
 DOMAIN 558 765 PI3K/PI4K.
 MOD_RES 258 258 Phosphoserine.
 MOD_RES 263 263 Phosphothreonine.
 MOD_RES 266 266 Phosphoserine.
 MOD_RES 277 277 Phosphoserine.
 MOD_RES 294 294 Phosphoserine.
 MOD_RES 428 428 Phosphoserine.
 MOD_RES 438 438 Phosphothreonine.
 MOD_RES 511 511 Phosphoserine.
 MOD_RES 519 519 Phosphothreonine.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Endoplasmic reticulum; Golgi apparatus; Kinase; Membrane; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 816 AA 
Protein Sequence
MGDTVVEPAP LKPTSEPTSG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK 60
LLHGGVAVSS RGTPLELVNG DGVDSEIRCL DDPPAQIREE EDEMGAAVAS GTAKGARRRR 120
QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL 180
NMYIHMDEDV GDAIKPYIVH RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI 240
LSDELKPAHR KRELPSLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 300
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ RLISELSLLN 360
HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY VEVLECENFD TTSVPARIPE 420
NRIRSTRSVE NLPECGITHE QRAGSFSTVP NYDNDDEAWS VDDIGELQVE LPEVHTNSCD 480
NISQFSVDSI TSQESKEPVF IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK 540
VRRIREGSPY GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI 600
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ RNFVQSCAGY 660
CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN LGFETSAFKL TTEFVDVMGG 720
LDGDMFNYYK MLMLQGLIAA RKHMDKVVQI VEIMQQGSQL PCFHGSSTIR NLKERFHMSM 780
TEEQLQLLVE QMVDGSMRSI TTKLYDGFQY LTNGIM 816 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005768; C:endosome; TAS:ProtInc.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030867; C:rough endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0004430; F:1-phosphatidylinositol 4-kinase activity; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:InterPro.
 GO:0006898; P:receptor-mediated endocytosis; TAS:ProtInc.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR027003; Phosphatidylino_kinase.
 IPR000403; PI3/4_kinase_cat_dom.
 IPR018936; PI3/4_kinase_CS.
 IPR015433; PI_Kinase.
 IPR001263; PInositide-3_kin_accessory_dom. 
Pfam
 PF00454; PI3_PI4_kinase 
SMART
 SM00146; PI3Kc 
PROSITE
 PS00915; PI3_4_KINASE_1
 PS00916; PI3_4_KINASE_2
 PS50290; PI3_4_KINASE_3
 PS51545; PIK_HELICAL 
PRINTS