CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001806
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Complement factor D 
Protein Synonyms/Alias
 Adipsin; C3 convertase activator; Properdin factor D 
Gene Name
 CFD 
Gene Synonyms/Alias
 DF; PFD 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
92LSQPEPSKRLYDVLRubiquitination[1, 2, 3, 4]
123LLLQLSEKATLGPAVubiquitination[2]
205SNRRDSCKGDSGGPLubiquitination[3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. 
Sequence Annotation
 DOMAIN 26 253 Peptidase S1.
 ACT_SITE 66 66 Charge relay system.
 ACT_SITE 114 114 Charge relay system.
 ACT_SITE 208 208 Charge relay system.
 DISULFID 51 67
 DISULFID 148 214
 DISULFID 179 195
 DISULFID 204 229  
Keyword
 3D-structure; Complement alternate pathway; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Hydrolase; Immunity; Innate immunity; Polymorphism; Protease; Reference proteome; Secreted; Serine protease; Signal; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 253 AA 
Protein Sequence
MHSWERLAVL VLLGAAACAA PPRGRILGGR EAEAHARPYM ASVQLNGAHL CGGVLVAEQW 60
VLSAAHCLED AADGKVQVLL GAHSLSQPEP SKRLYDVLRA VPHPDSQPDT IDHDLLLLQL 120
SEKATLGPAV RPLPWQRVDR DVAPGTLCDV AGWGIVNHAG RRPDSLQHVL LPVLDRATCN 180
RRTHHDGAIT ERLMCAESNR RDSCKGDSGG PLVCGGVLEG VVTSGSRVCG NRKKPGIYTR 240
VASYAAWIDS VLA 253 
Gene Ontology
 GO:0005576; C:extracellular region; TAS:Reactome.
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0031093; C:platelet alpha granule lumen; TAS:Reactome.
 GO:0004252; F:serine-type endopeptidase activity; TAS:ProtInc.
 GO:0006957; P:complement activation, alternative pathway; TAS:Reactome.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0002576; P:platelet degranulation; TAS:Reactome.
 GO:0006508; P:proteolysis; TAS:ProtInc. 
Interpro
 IPR001254; Peptidase_S1.
 IPR018114; Peptidase_S1_AS.
 IPR001314; Peptidase_S1A.
 IPR009003; Trypsin-like_Pept_dom. 
Pfam
 PF00089; Trypsin 
SMART
 SM00020; Tryp_SPc 
PROSITE
 PS50240; TRYPSIN_DOM
 PS00134; TRYPSIN_HIS
 PS00135; TRYPSIN_SER 
PRINTS
 PR00722; CHYMOTRYPSIN.