UniProt Accession

 PUR9_MOUSE; Q9CWJ9; Q3UTQ3; Q80UH0; Q8BPF0; Q8BQV9; Q9CRI1; Q9CZW9 

Genbank Protein ID

 AK010449; AK010611; AK012074; AK046353; AK076091; AK139235; BC039925 

Genbank Nucleotide ID

 BAB26949.3; BAB27060.1; BAB28011.1; BAC32688.1; BAC36175.1; BAE23927.1; AAH39925.2 

Protein Name

 Bifunctional purine biosynthesis protein PURH 

Protein Synonyms/Alias

 Phosphoribosylaminoimidazolecarboxamide formyltransferase; 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase 

Gene Name

 Atic 

Gene Synonyms/Alias

 Purh 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
14	ALFSVSDKTGLVEFA	ubiquitination	[1]
66	EMLGGRVKTLHPAVH	ubiquitination	[1]
199	YFRKQYSKGISQMPL	ubiquitination	[1]
223	PAQLYTLKPKLPITV	acetylation	[2]
223	PAQLYTLKPKLPITV	ubiquitination	[1]
266	IPAAASFKHVSPAGA	acetylation	[2, 3, 4]
352	GYEEEALKILSKKKN	acetylation	[3]
352	GYEEEALKILSKKKN	ubiquitination	[1]
397	RNNGVVDKSLFSNIV	ubiquitination	[1]
406	LFSNIVTKNKDLPES	ubiquitination	[1]
437	SNSVCYAKDGQVIGI	acetylation	[2]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis (By similarity). 

Sequence Annotation

 NP_BIND 12 14 IMP (By similarity).
 NP_BIND 34 37 IMP (By similarity).
 NP_BIND 64 67 IMP (By similarity).
 NP_BIND 101 104 IMP (By similarity).
 NP_BIND 125 127 IMP (By similarity).
 REGION 207 208 AICAR binding (By similarity).
 ACT_SITE 137 137 Proton acceptor (Potential).
 ACT_SITE 267 267 Proton acceptor (Probable).
 BINDING 316 316 AICAR; via carbonyl oxygen (By
 BINDING 339 339 AICAR (By similarity).
 BINDING 431 431 AICAR; shared with dimeric partner (By
 BINDING 451 451 AICAR; shared with dimeric partner (By
 BINDING 541 541 AICAR; via carbonyl oxygen; shared with
 BINDING 588 588 AICAR; shared with dimeric partner (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 199 199 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Hydrolase; Multifunctional enzyme; Purine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 592 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0003937; F:IMP cyclohydrolase activity; IEA:EC.
 GO:0004643; F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity; IEA:EC.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0003360; P:brainstem development; IEA:Compara.
 GO:0021549; P:cerebellum development; IEA:Compara.
 GO:0021987; P:cerebral cortex development; IEA:Compara.
 GO:0046452; P:dihydrofolate metabolic process; IEA:Compara.
 GO:0009116; P:nucleoside metabolic process; IEA:Compara.
 GO:0031100; P:organ regeneration; IEA:Compara.
 GO:0010035; P:response to inorganic substance; IEA:Compara.
 GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:Compara. 

Interpro

 IPR024051; AICAR_Tfase_dom.
 IPR024050; AICAR_Tfase_insert_dom.
 IPR002695; AICARFT_IMPCHas.
 IPR016193; Cytidine_deaminase-like.
 IPR011607; MGS-like_dom. 

Pfam

 PF01808; AICARFT_IMPCHas
 PF02142; MGS 

SMART

 SM00798; AICARFT_IMPCHas
 SM00851; MGS 

PROSITE

  

PRINTS