CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006994
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Class E vacuolar protein-sorting machinery protein HSE1 
Protein Synonyms/Alias
  
Gene Name
 HSE1 
Gene Synonyms/Alias
 YHL002W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
14KIRNALLKATDPKLRubiquitination[1]
122KQLSDSFKDDPSLRAubiquitination[1]
152VQPNVPEKHNMSTQAubiquitination[1]
189KKLQEQEKESAEVLPubiquitination[1, 2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane. 
Sequence Annotation
 DOMAIN 15 145 VHS.
 REPEAT 162 181 UIM.
 DOMAIN 217 276 SH3.
 MOD_RES 162 162 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Endosome; Membrane; Phosphoprotein; Protein transport; Reference proteome; SH3 domain; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 452 AA 
Protein Sequence
MSSSAIKIRN ALLKATDPKL RSDNWQYILD VCDLVKEDPE DNGQEVMSLI EKRLEQQDAN 60
VILRTLSLTV SLAENCGSRL RQEISSKNFT SLLYALIESH SVHITLKKAV TDVVKQLSDS 120
FKDDPSLRAM GDLYDKIKRK APYLVQPNVP EKHNMSTQAD NSDDEELQKA LKMSLFEYEK 180
QKKLQEQEKE SAEVLPQQQQ QHQQQNQAPA HKIPAQTVVR RVRALYDLTT NEPDELSFRK 240
GDVITVLEQV YRDWWKGALR GNMGIFPLNY VTPIVEPSKE EIEKEKNKEA IVFSQKTTID 300
QLHNSLNAAS KTGNSNEVLQ DPHIGDMYGS VTPLRPQVTR MLGKYAKEKE DMLSLRQVLA 360
NAERSYNQLM DRAANAHISP PVPGPALYAG MTHANNTPVM PPQRQSYQSN EYSPYPSNLP 420
IQHPTNSANN TPQYGYDLGY SVVSQPPPGY EQ 452 
Gene Ontology
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0033565; C:ESCRT-0 complex; IPI:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0045324; P:late endosome to vacuole transport; IMP:SGD.
 GO:0006623; P:protein targeting to vacuole; IMP:SGD. 
Interpro
 IPR008942; ENTH_VHS.
 IPR000108; p67phox.
 IPR001452; SH3_domain.
 IPR027423; Sorting_HSE1.
 IPR003903; Ubiquitin-int_motif.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 
Pfam
 PF00018; SH3_1
 PF00790; VHS 
SMART
 SM00326; SH3
 SM00726; UIM
 SM00288; VHS 
PROSITE
 PS50002; SH3
 PS50330; UIM
 PS50179; VHS 
PRINTS
 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.