UniProt Accession

 NDKB_RAT; P19804 

Genbank Protein ID

 M55331; M91597; BC086599 

Genbank Nucleotide ID

 AAA41684.1; AAA42017.1; AAH86599.1 

Protein Name

 Nucleoside diphosphate kinase B 

Protein Synonyms/Alias

 NDK B; NDP kinase B; Histidine protein kinase NDKB; P18 

Gene Name

 Nme2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
12	ERTFIAIKPDGVQRG	ubiquitination	[1]
31	IIKRFEQKGFRLVAM	acetylation	[2]
39	GFRLVAMKFLRASEE	acetylation	[2]
49	RASEEHLKQHYIDLK	acetylation	[2]
49	RASEEHLKQHYIDLK	ubiquitination	[1]
56	KQHYIDLKDRPFFPG	acetylation	[2]
56	KQHYIDLKDRPFFPG	ubiquitination	[1]
100	ETNPADSKPGTIRGD	acetylation	[2]
100	ETNPADSKPGTIRGD	ubiquitination	[1]
128	DSVESAEKEIGLWFK	acetylation	[2]
135	KEIGLWFKPEELIDY	acetylation	[2]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC. Exhibits histidine protein kinase activity (By similarity). 

Sequence Annotation

 REGION 2 66 Interaction with AKAP13 (By similarity).
 ACT_SITE 118 118 Pros-phosphohistidine intermediate (By
 BINDING 12 12 ATP (By similarity).
 BINDING 60 60 ATP (By similarity).
 BINDING 88 88 ATP (By similarity).
 BINDING 94 94 ATP (By similarity).
 BINDING 105 105 ATP (By similarity).
 BINDING 115 115 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 94 94 Phosphothreonine (By similarity).
 MOD_RES 128 128 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide metabolism; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 152 AA 

Protein Sequence

Gene Ontology

 GO:0071944; C:cell periphery; ISS:UniProtKB.
 GO:0030027; C:lamellipodium; ISS:HGNC.
 GO:0031966; C:mitochondrial membrane; IDA:RGD.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:RGD.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0001726; C:ruffle; ISS:HGNC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008144; F:drug binding; IPI:RGD.
 GO:0005504; F:fatty acid binding; IPI:RGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004550; F:nucleoside diphosphate kinase activity; IDA:RGD.
 GO:0004673; F:protein histidine kinase activity; IEA:EC.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:RGD.
 GO:0071398; P:cellular response to fatty acid; IEP:RGD.
 GO:0071333; P:cellular response to glucose stimulus; IEP:RGD.
 GO:0034599; P:cellular response to oxidative stress; IEP:RGD.
 GO:0006241; P:CTP biosynthetic process; IDA:RGD.
 GO:0006183; P:GTP biosynthetic process; IDA:RGD.
 GO:0007229; P:integrin-mediated signaling pathway; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; ISS:HGNC.
 GO:0002762; P:negative regulation of myeloid leukocyte differentiation; IDA:RGD.
 GO:0030819; P:positive regulation of cAMP biosynthetic process; IMP:RGD.
 GO:0050679; P:positive regulation of epithelial cell proliferation; ISS:HGNC.
 GO:0045618; P:positive regulation of keratinocyte differentiation; ISS:HGNC.
 GO:0010976; P:positive regulation of neuron projection development; IMP:RGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:RGD.
 GO:0051259; P:protein oligomerization; IDA:RGD.
 GO:0060416; P:response to growth hormone stimulus; IEP:RGD.
 GO:0006228; P:UTP biosynthetic process; IDA:RGD. 

Interpro

 IPR001564; Nucleoside_diP_kinase.
 IPR023005; Nucleoside_diP_kinase_AS. 

Pfam

 PF00334; NDK 

SMART

 SM00562; NDK 

PROSITE

 PS00469; NDP_KINASES 

PRINTS

 PR01243; NUCDPKINASE.