UniProt Accession

 ECI2_RAT; Q5XIC0 

Genbank Protein ID

 BC083764 

Genbank Nucleotide ID

 AAH83764.1 

Protein Name

 Enoyl-CoA delta isomerase 2, mitochondrial 

Protein Synonyms/Alias

 Delta(3),delta(2)-enoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase; Peroxisomal 3,2-trans-enoyl-CoA isomerase; pECI 

Gene Name

 Eci2 

Gene Synonyms/Alias

 Peci 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
49	ENAMNQVKLLKKDPG	acetylation	[1]
52	MNQVKLLKKDPGNEV	acetylation	[1]
60	KDPGNEVKLRLYALY	acetylation	[1]
79	EGPCTMPKPGVFDFV	acetylation	[1]
90	FDFVNKAKWDAWNAL	acetylation	[1]
138	DGKAQESKGILVTSE	acetylation	[1]
336	TRLKTYAKLPPNSMR	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. 

Sequence Annotation

 DOMAIN 37 122 ACB.
 REGION 64 68 Acyl-CoA binding (By similarity).
 REGION 149 319 ECH-like.
 MOTIF 389 391 Microbody targeting signal (Potential).
 BINDING 90 90 Acyl-CoA (By similarity).
 BINDING 109 109 Acyl-CoA (By similarity).
 MOD_RES 49 49 N6-acetyllysine (By similarity).
 MOD_RES 60 60 N6-acetyllysine (By similarity).
 MOD_RES 90 90 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Isomerase; Mitochondrion; Peroxisome; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 391 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0005782; C:peroxisomal matrix; IEA:UniProtKB-SubCell.
 GO:0005777; C:peroxisome; IDA:RGD.
 GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; IEA:EC.
 GO:0000062; F:fatty-acyl-CoA binding; IEA:InterPro.
 GO:0016863; F:intramolecular oxidoreductase activity, transposing C=C bonds; IDA:RGD.
 GO:0006635; P:fatty acid beta-oxidation; IDA:RGD. 

Interpro

 IPR022408; Acyl-CoA-binding_prot_CS.
 IPR000582; Acyl-CoA-binding_protein.
 IPR001753; Crotonase_core_superfam.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx. 

Pfam

 PF00887; ACBP
 PF00378; ECH 

SMART

  

PROSITE

 PS00880; ACB_1
 PS51228; ACB_2 

PRINTS

 PR00689; ACOABINDINGP.