CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020604
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exosome complex exonuclease RRP44 
Protein Synonyms/Alias
 Protein DIS3 homolog; Ribosomal RNA-processing protein 44 
Gene Name
 Dis3 
Gene Synonyms/Alias
 Kiaa1008; Rrp44 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
18TRAGGVVKIVREHYLacetylation[1, 2]
236SEHLPLSKLQQGIKSubiquitination[3]
242SKLQQGIKSGSYLQGubiquitination[3]
922SSNLQHQKIRMALVEacetylation[4]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities (By similarity). 
Sequence Annotation
 DOMAIN 64 182 PINc.
 MOD_RES 18 18 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Endonuclease; Exonuclease; Exosome; Hydrolase; Magnesium; Manganese; Nuclease; Nucleus; Reference proteome; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 958 AA 
Protein Sequence
MLRSKTFLKK TRAGGVVKIV REHYLRDDIG CGAPACSACG GAHAGPALEL QPRDQASSLC 60
PWPHYLLPDT NVLLHQIDVL EHPAIRNVIV LQTVMQEVRN RSAPIYKRIR DVTNNQEKHF 120
YTFTNEHHKE TYIEQEQGEN ANDRNDRAIR VAAKWYNEHL KRVAADSQLQ VILITNDRKN 180
KEKAVQEGIP AFTCEEYVKS LTANPELIDR LAYLSDEMNE IESGKIIFSE HLPLSKLQQG 240
IKSGSYLQGT FRASRENYLE ATVWIHGDKE EEKEILIQGI KHLNRAVHED IVAVELLPRS 300
QWVAPSSVVL DDEGQNEDDV EKDEERELLL KTAVSEKMLR PTGRVVGIIK RNWRPYCGML 360
SKSDIKESRR HLFTPADKRI PRIRIETRQA SALEGRRIIV AIDGWPRNSR YPNGHFVKNL 420
GDVGEKETET EVLLLEHDVP HQPFSQAVLS FLPRMPWSIT EEDMKNREDL RHLCVCSVDP 480
PGCTDIDDAL HCRELSNGNL EVGVHIADVS HFIRPGNALD QESARRGTTV YLCEKRIDMV 540
PELLSSNLCS LRSNVDRLAF SCIWEMNHNA EILKTRFTKS VINSKASLTY AEAQMRIDSA 600
AMNDDITTSL RGLNQLAKIL KKGRIEKGAL TLSSPEIRFH MDSETHDPID LQTKELRETN 660
SMVEEFMLLA NISVAKKIHE EFSEHALLRK HPAPPPSNYD ILVKAAKSKN LQIKTDTAKS 720
LADSLDRAES PDFPYLNTLL RILATRCMMQ AVYFCSGMDN DFHHYGLASP IYTHFTSPIR 780
RYADIIVHRL LAVAIGADCT YPELTDKHKL SDICKNLNFR HKMAQYAQRA SVAFHTQLFF 840
KSKGIVSEEA YILFVRKNAI VVLIPKYGLE GTVFFEEKDK PKPRLAYDDE IPSLRIEGTV 900
FHVFDKVKVK ITLDSSNLQH QKIRMALVEP QIPGINIPPN VADKALTAPG GKKRKLEK 958 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000176; C:nuclear exosome (RNase complex); ISS:UniProtKB.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; ISS:UniProtKB.
 GO:0000175; F:3'-5'-exoribonuclease activity; ISS:UniProtKB.
 GO:0004519; F:endonuclease activity; ISS:UniProtKB.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:Compara.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0071034; P:CUT catabolic process; IEA:Compara.
 GO:0016075; P:rRNA catabolic process; IEA:Compara.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR002716; PIN_dom.
 IPR006596; PINc_nuc-bd.
 IPR001900; RNase_II/R.
 IPR022966; RNase_II/R_CS. 
Pfam
 PF13638; PIN_4
 PF00773; RNB 
SMART
 SM00670; PINc
 SM00955; RNB 
PROSITE
 PS01175; RIBONUCLEASE_II 
PRINTS