CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017999
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ceramide kinase 
Protein Synonyms/Alias
 hCERK; Acylsphingosine kinase; Lipid kinase 4; LK4 
Gene Name
 CERK 
Gene Synonyms/Alias
 KIAA1646 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
473EDEDSDLKEGGKKRFubiquitination[1, 2]
477SDLKEGGKKRFGHICubiquitination[1]
478DLKEGGKKRFGHICSubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. 
Sequence Annotation
 DOMAIN 128 278 DAGKc.
 REGION 1 125 Required for binding to sulfatide and  
Keyword
 ATP-binding; Calcium; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Nucleotide-binding; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 537 AA 
Protein Sequence
MGATGAAEPL QSVLWVKQQR CAVSLEPARA LLRWWRSPGP GAGAPGADAC SVPVSEIIAV 60
EETDVHGKHQ GSGKWQKMEK PYAFTVHCVK RARRHRWKWA QVTFWCPEEQ LCHLWLQTLR 120
EMLEKLTSRP KHLLVFINPF GGKGQGKRIY ERKVAPLFTL ASITTDIIVT EHANQAKETL 180
YEINIDKYDG IVCVGGDGMF SEVLHGLIGR TQRSAGVDQN HPRAVLVPSS LRIGIIPAGS 240
TDCVCYSTVG TSDAETSALH IVVGDSLAMD VSSVHHNSTL LRYSVSLLGY GFYGDIIKDS 300
EKKRWLGLAR YDFSGLKTFL SHHCYEGTVS FLPAQHTVGS PRDRKPCRAG CFVCRQSKQQ 360
LEEEQKKALY GLEAAEDVEE WQVVCGKFLA INATNMSCAC RRSPRGLSPA AHLGDGSSDL 420
ILIRKCSRFN FLRFLIRHTN QQDQFDFTFV EVYRVKKFQF TSKHMEDEDS DLKEGGKKRF 480
GHICSSHPSC CCTVSNSSWN CDGEVLHSPA IEVRVHCQLV RLFARGIEEN PKPDSHS 537 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016021; C:integral to membrane; IDA:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0001729; F:ceramide kinase activity; IDA:UniProtKB.
 GO:0004143; F:diacylglycerol kinase activity; IEA:InterPro.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0006672; P:ceramide metabolic process; IDA:UniProtKB.
 GO:0006687; P:glycosphingolipid metabolic process; TAS:Reactome.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR001206; Diacylglycerol_kinase_cat_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF00781; DAGK_cat 
SMART
 SM00046; DAGKc
 SM00233; PH 
PROSITE
 PS50146; DAGK 
PRINTS