UniProt Accession

 GRAP2_HUMAN; O75791; O43726; Q9NRB7 

Genbank Protein ID

 AF102694; AF129476; AF042380; AF236119; AF236120; AF121002; AY069959; Y18051; AJ011736; AF090456; CR456498; Z82206; BC025692; BC026002 

Genbank Nucleotide ID

 AAD04926.1; AAD41782.1; AAC69273.1; AAF60319.1; AAF60320.1; AAF31758.1; AAL58573.1; CAA77021.1; CAA09757.1; AAD13027.1; CAG30384.1; CAI42713.1; AAH25692.1; AAH26002.1 

Protein Name

 GRB2-related adapter protein 2 

Protein Synonyms/Alias

 Adapter protein GRID; GRB-2-like protein; GRB2L; GRBLG; GRBX; Grf40 adapter protein; Grf-40; Growth factor receptor-binding protein; Hematopoietic cell-associated adapter protein GrpL; P38; Protein GADS; SH3-SH2-SH3 adapter Mona 

Gene Name

 GRAP2 

Gene Synonyms/Alias

 GADS; GRB2L; GRID 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
36	SNQEEWFKAELGSQE	ubiquitination	[1]
48	SQEGYVPKNFIDIQF	ubiquitination	[1]
57	FIDIQFPKWFHEGLS	ubiquitination	[1]
106	EDDVQHFKVMRDNKG	acetylation	[2]
112	FKVMRDNKGNYFLWT	ubiquitination	[1]
121	NYFLWTEKFPSLNKL	ubiquitination	[1]
127	EKFPSLNKLVDYYRT	ubiquitination	[1]
141	TNSISRQKQIFLRDR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. 

Sequence Annotation

 DOMAIN 1 56 SH3 1.
 DOMAIN 58 149 SH2.
 DOMAIN 271 330 SH3 2.
 MOD_RES 45 45 Phosphotyrosine.
 MOD_RES 106 106 N6-acetyllysine.
 MOD_RES 262 262 Phosphothreonine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Endosome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; SH2 domain; SH3 domain. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 330 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005768; C:endosome; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
 GO:0007267; P:cell-cell signaling; TAS:ProtInc.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0007265; P:Ras protein signal transduction; TAS:ProtInc.
 GO:0031295; P:T cell costimulation; TAS:Reactome.
 GO:0050852; P:T cell receptor signaling pathway; TAS:Reactome. 

Interpro

 IPR000980; SH2.
 IPR001452; SH3_domain. 

Pfam

 PF00017; SH2
 PF00018; SH3_1 

SMART

 SM00252; SH2
 SM00326; SH3 

PROSITE

 PS50001; SH2
 PS50002; SH3 

PRINTS

 PR00401; SH2DOMAIN.
 PR00452; SH3DOMAIN.