CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009033
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytochrome b5 
Protein Synonyms/Alias
  
Gene Name
 Cyb5a 
Gene Synonyms/Alias
 Cyb5 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
7*MAGQSDKDVKYYTLacetylation[1]
7*MAGQSDKDVKYYTLubiquitination[2]
10GQSDKDVKYYTLEEIacetylation[1]
10GQSDKDVKYYTLEEIubiquitination[2]
19YTLEEIQKHKDSKSTacetylation[1, 3, 4]
19YTLEEIQKHKDSKSTubiquitination[2]
24IQKHKDSKSTWVILHubiquitination[2]
33TWVILHHKVYDLTKFacetylation[1]
39HKVYDLTKFLEEHPGacetylation[1]
39HKVYDLTKFLEEHPGubiquitination[2]
77TDARELSKTYIIGELacetylation[1]
77TDARELSKTYIIGELubiquitination[2]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [4] Proteomics of mitochondrial inner and outer membranes.
 Distler AM, Kerner J, Hoppel CL.
 Proteomics. 2008 Oct;8(19):4066-82. [PMID: 18763707] 
Functional Description
 Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-5 double bond introduction during the C-5 desaturation. 
Sequence Annotation
 DOMAIN 9 85 Cytochrome b5 heme-binding.
 METAL 44 44 Iron (heme axial ligand) (By similarity).
 METAL 68 68 Iron (heme axial ligand) (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 19 19 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Electron transport; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 134 AA 
Protein Sequence
MAGQSDKDVK YYTLEEIQKH KDSKSTWVIL HHKVYDLTKF LEEHPGGEEV LREQAGGDAT 60
ENFEDVGHST DARELSKTYI IGELHPDDRS KIAKPSDTLI TTVESNSSWW TNWVIPAISA 120
LAVALMYRLY MAED 134 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004768; F:stearoyl-CoA 9-desaturase activity; TAS:MGI.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0006631; P:fatty acid metabolic process; IC:MGI. 
Interpro
 IPR001199; Cyt_B5-like_heme/steroid-bd.
 IPR018506; Cyt_B5_heme-BS. 
Pfam
 PF00173; Cyt-b5 
SMART
  
PROSITE
 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 
PRINTS
 PR00363; CYTOCHROMEB5.