CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030954
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cysteine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 cDNA FLJ59563, highly similar to Cysteinyl-tRNA synthetase (EC 6.1.1.16) 
Gene Name
 CARS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
52YNSLTRNKEVFIPQDubiquitination[1]
61VFIPQDGKKVTWYCCubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 Aminoacyl-tRNA synthetase; Complete proteome; Ligase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 739 AA 
Protein Sequence
MEPTSGPFRT DLGYHLVGCS SGKGRRVQPQ WSPPAGTQPC RLHLYNSLTR NKEVFIPQDG 60
KKVTWYCCGP TVYDASHMGH ARSYISFDIL RRVLKDYFKF DVFYCMNITD IDDKIIKRAR 120
QNHLFEQYRE KRPEAAQLLE DVQAALKPFS VKLNETTDPD KKQMLERIQH AVQLATEPLE 180
KAVQSRLTGE EVNSCVEVLL EEAKDLLSDW LDSTLGCDVT DNSIFSKLPK FWEGDFHRDM 240
EALNVLPPDV LTRVSEYVPE IVNFVQKIVD NGYGYVSNGS VYFDTAKFAS SEKHSYGKLV 300
PEAVGDQKAL QEGEGDLSIS ADRLSEKRSP NDFALWKASK PGEPSWPCPW GKGRPGWHIE 360
CSAMAGTLLG ASMDIHGGGF DLRFPHHDNE LAQSEAYFEN DCWVRYFLHT GHLTIAGCKM 420
SKSLKNFITI KDALKKHSAR QLRLAFLMHS WKDTLDYSSN TMESALQYEK FLNEFFLNVK 480
DILRAPVDIT GQFEKWGEEE AELNKNFYDK KTAIHKALCD NVDTRTVMEE MRALVSQCNL 540
YMAARKAVRK RPNQALLENI ALYLTHMLKI FGAVEEDSSL GFPVGGPGTS LSLEATVMPY 600
LQVLSEFREG VRKIAREQKV PEILQLSDAL RDNILPELGV RFEDHEGLPT VVKLVDRNTL 660
LKEREEKRRV EEEKRKKKEE AARRKQEQEA AKLAKMKIPP SEMFLSETDK YSKFDENVSM 720
VCPHMTWRAK SSAKGKPRS 739 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004817; F:cysteine-tRNA ligase activity; IEA:InterPro.
 GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR015803; Cys-tRNA-ligase.
 IPR024909; Cys-tRNA/MSH_ligase.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 
Pfam
 PF01406; tRNA-synt_1e 
SMART
  
PROSITE
  
PRINTS
 PR00983; TRNASYNTHCYS.