CPLM-007777

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-007777

UniProt Accession

SYT2_MOUSE; P46097; Q8R0E1

Genbank Protein ID

D37793; AF257303; AF257304; AK036357; BC027019

Genbank Nucleotide ID

BAA07041.1; AAF68987.1; AAF68988.1; BAC29397.1; AAH27019.1

Protein Name

Synaptotagmin-2

Protein Synonyms/Alias

Synaptotagmin II; SytII

Gene Name

Syt2

Gene Synonyms/Alias

Created Date

July 27, 2013

Organism

Mus musculus (Mouse)

NCBI Taxa ID

10090

Lysine Modification

Position	Peptide	Type	References
112	MKNAMNMKDMKGGQD	ubiquitination	[1]
268	RDLQGGEKEEPEKLG	ubiquitination	[1]
289	RYVPTAGKLTVCILE	ubiquitination	[1]
298	TVCILEAKNLKKMDV	ubiquitination	[1]
367	VTVLDYDKLGKNEAI	ubiquitination	[1]
376	GKNEAIGKIFVGSNA	ubiquitination	[1]

Reference

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.

Sequence Annotation

DOMAIN 156 245 C2 1.
DOMAIN 287 378 C2 2.
REGION 136 382 Phospholipid binding (Probable).
METAL 172 172 Calcium 2; via carbonyl oxygen (By
METAL 173 173 Calcium 1 (By similarity).
METAL 173 173 Calcium 2 (By similarity).
METAL 179 179 Calcium 1 (By similarity).
METAL 231 231 Calcium 1 (By similarity).
METAL 231 231 Calcium 2 (By similarity).
METAL 232 232 Calcium 1; via carbonyl oxygen (By
METAL 233 233 Calcium 1 (By similarity).
METAL 233 233 Calcium 2 (By similarity).
METAL 233 233 Calcium 3 (By similarity).
METAL 236 236 Calcium 3 (By similarity).
METAL 237 237 Calcium 3; via carbonyl oxygen (By
METAL 239 239 Calcium 2 (By similarity).
METAL 239 239 Calcium 3 (By similarity).
CARBOHYD 32 32 N-linked (GlcNAc...) (Potential).

Keyword

3D-structure; Calcium; Cell junction; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Glycoprotein; Membrane; Metal-binding; Reference proteome; Repeat; Synapse; Transmembrane; Transmembrane helix.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

422 AA

Protein Sequence

MRNIFKRNQE PNVAPATTTA TMPLAPVAPA DNSTESTGPG ESQEDMFAKL KEKFFNEINK 60
IPLPPWALIA MAVVAGLLLL TCCFCICKKC CCKKKKNKKE KGKGMKNAMN MKDMKGGQDD 120
DDAETGLTEG EGEGEEEKEP ENLGKLQFSL DYDFQANQLT VGVLQAAELP ALDMGGTSDP 180
YVKVFLLPDK KKKYETKVHR KTLNPAFNET FTFKVPYQEL AGKTLVMAIY DFDRFSKHDI 240
IGEVKVPMNT VDLGQPIEEW RDLQGGEKEE PEKLGDICTS LRYVPTAGKL TVCILEAKNL 300
KKMDVGGLSD PYVKIHLMQN GKRLKKKKTT VKKKTLNPYF NESFSFEIPF EQIQKVQVVV 360
TVLDYDKLGK NEAIGKIFVG SNATGTELRH WSDMLANPRR PIAQWHSLKP EEEVDALLGK 420
NK 422

Gene Ontology

GO:0030054; C:cell junction; IEA:UniProtKB-KW.
GO:0042584; C:chromaffin granule membrane; IEA:UniProtKB-SubCell.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005886; C:plasma membrane; IDA:MGI.
GO:0030672; C:synaptic vesicle membrane; IEA:UniProtKB-SubCell.
GO:0005509; F:calcium ion binding; IDA:MGI.
GO:0005544; F:calcium-dependent phospholipid binding; IDA:MGI.
GO:0005215; F:transporter activity; IEA:InterPro.

Interpro

IPR000008; C2_Ca-dep.
IPR008973; C2_Ca/lipid-bd_dom_CaLB.
IPR020477; C2_dom.
IPR018029; C2_membr_targeting.
IPR001565; Synaptotagmin.

Pfam

PF00168; C2

SMART

SM00239; C2

PROSITE

PS50004; C2

PRINTS

PR00360; C2DOMAIN.
PR00399; SYNAPTOTAGMN.