UniProt Accession

 DOHH_HUMAN; Q9BU89; O75265 

Genbank Protein ID

 AC005551; AC093052; BC002817; BC009863 

Genbank Nucleotide ID

 AAC33193.1; AAH02817.1; AAH09863.1 

Protein Name

 Deoxyhypusine hydroxylase 

Protein Synonyms/Alias

 hDOHH; Deoxyhypusine dioxygenase; Deoxyhypusine monooxygenase; HEAT-like repeat-containing protein 1 

Gene Name

 DOHH 

Gene Synonyms/Alias

 HLRC1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
18	GQTLVDPKQPLQARF	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)- L-lysine intermediate to form hypusine, an essential post- translational modification only found in mature eIF-5A factor. 

Sequence Annotation

 REPEAT 54 80 HEAT-like PBS-type 1.
 REPEAT 87 113 HEAT-like PBS-type 2.
 REPEAT 174 200 HEAT-like PBS-type 3.
 REPEAT 205 231 HEAT-like PBS-type 4.
 REPEAT 238 264 HEAT-like PBS-type 5.
 METAL 56 56 Iron 1.
 METAL 57 57 Iron 1.
 METAL 89 89 Iron 1.
 METAL 90 90 Iron 1.
 METAL 207 207 Iron 2.
 METAL 208 208 Iron 2.
 METAL 240 240 Iron 2.
 METAL 241 241 Iron 2.  

Keyword

 Complete proteome; Direct protein sequencing; Hypusine biosynthesis; Iron; Metal-binding; Monooxygenase; Oxidoreductase; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 302 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0019135; F:deoxyhypusine monooxygenase activity; IMP:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008612; P:peptidyl-lysine modification to hypusine; IMP:UniProtKB.
 GO:0043687; P:post-translational protein modification; TAS:Reactome. 

Interpro

 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR027517; Deoxyhypusine_hydroxylase.
 IPR021133; HEAT_type_2.
 IPR004155; PBS_lyase_HEAT. 

Pfam

  

SMART

 SM00567; EZ_HEAT 

PROSITE

 PS50077; HEAT_REPEAT 

PRINTS