CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007942
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-actin-capping protein subunit alpha-1 
Protein Synonyms/Alias
 CapZ alpha-1 
Gene Name
 Capza1 
Gene Synonyms/Alias
 Cappa1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
19EKVRIAAKFITHAPPacetylation[1, 2, 3]
97PRNQISFKFDHLRKEacetylation[1, 3]
268QLPVTRTKIDWNKILubiquitination[4]
273RTKIDWNKILSYKIGacetylation[1, 2, 3]
273RTKIDWNKILSYKIGubiquitination[4, 5]
278WNKILSYKIGKEMQNacetylation[2]
278WNKILSYKIGKEMQNubiquitination[5]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
 Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A.
 Nature. 2012 Nov 22;491(7425):618-21. [PMID: 23086144]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 19 19 N6-acetyllysine (By similarity).
 MOD_RES 97 97 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Actin capping; Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 286 AA 
Protein Sequence
MADFEDRVSD EEKVRIAAKF ITHAPPGEFN EVFNDVRLLL NNDNLLREGA AHAFAQYNMD 60
QFTPVKIEGY DDQVLITEHG DLGNSRFLDP RNQISFKFDH LRKEASDPQP EDVDGGLKSW 120
RESCDSALRA YVKDHYSNGF CTVYAKTIDG QQTIIACIES HQFQPKNFWN GRWRSEWKFT 180
ITPPSAQVVG VLKIQVHYYE DGNVQLVSHK DVQDSVTVSN EVQTTKEFIK IIESAENEYQ 240
TAISENYQTM SDTTFKALRR QLPVTRTKID WNKILSYKIG KEMQNA 286 
Gene Ontology
 GO:0030863; C:cortical cytoskeleton; IDA:MGI.
 GO:0008290; C:F-actin capping protein complex; IDA:MGI.
 GO:0016020; C:membrane; IDA:MGI.
 GO:0071203; C:WASH complex; ISS:UniProtKB.
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
 GO:0051016; P:barbed-end actin filament capping; IDA:MGI. 
Interpro
 IPR017865; F-actin_cap_asu_CS.
 IPR002189; WASH_F-actin_cap_alpha. 
Pfam
 PF01267; F-actin_cap_A 
SMART
  
PROSITE
 PS00748; F_ACTIN_CAPPING_A_1
 PS00749; F_ACTIN_CAPPING_A_2 
PRINTS
 PR00191; FACTINCAPA.