CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016377
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial Rho GTPase 1 
Protein Synonyms/Alias
 MIRO-1; Ras homolog gene family member T1 
Gene Name
 Rhot1 
Gene Synonyms/Alias
 Arht1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
166ECSAKNLKNISELFYubiquitination[1]
195CPEEKEMKPACIKALubiquitination[1]
200EMKPACIKALTRIFKubiquitination[1]
207KALTRIFKISDQDNDubiquitination[1]
525EYCARIFKQHFMDSRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (By similarity). 
Sequence Annotation
 DOMAIN 11 190 Miro 1.
 DOMAIN 197 232 EF-hand 1.
 DOMAIN 317 352 EF-hand 2.
 DOMAIN 425 574 Miro 2.
 NP_BIND 24 31 GTP 1 (Potential).
 NP_BIND 70 74 GTP 1 (Potential).
 NP_BIND 131 134 GTP 1 (Potential).
 NP_BIND 438 445 GTP 2 (Potential).
 NP_BIND 476 480 GTP 2 (Potential).
 NP_BIND 540 543 GTP 2 (Potential).  
Keyword
 Alternative splicing; Calcium; Complete proteome; GTP-binding; Hydrolase; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Reference proteome; Repeat; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 631 AA 
Protein Sequence
MRAGRVRPLR ASDMKKDVRI LLVGEPRVGK TSLIMSLVSE EFPEEVPPRA EEITIPADVT 60
PERVPTHIVD YSEAEQSDEQ LHQEISQANV ICIVYAVNNK HSIDKVTSRW IPLINERTDK 120
DSRLPLILVG NKSDLVEYSS METILPIMNQ YTEIETCVEC SAKNLKNISE LFYYAQKAVL 180
HPTGPLYCPE EKEMKPACIK ALTRIFKISD QDNDGTLNDA ELNFFQRICF NTPLAPQALE 240
DVKNVVRKHL SDGVADSGLT LRGFLFLHTL FIQRGRHETT WTVLRRFGYD DDLDLTPEYL 300
FPLLKIPPDC TTELNHHAYL FLQSTFDKHD LDRDCALSPD ELKDLFQVFP YIPWGPDVNN 360
TVCTNERGWI TYQGFLSQWT LTTYLDVQRC LEYLGYLGYS ILTEQESQAS AITVTRDKKI 420
DLQKKQTQRN VFRCNVIGVK GCGKTGVLQS LLGRNLMRQK KIRDDHKSYY AINTVYVYGQ 480
EKYLLLHDIS ESEFLTEAET ICDVVCLVYD VTNPKSFEYC ARIFKQHFMD SRIPCLIVAA 540
KSDLHEVKQE HSISPTDFCR KHKMPPPQAF TCNTADAPSK DIFVKLTTMA MYPEDHYRGS 600
LSRDMGSTDR IENLRKIWVF LKTALHVTQA DLKSSTFWLR ASFGATVFAV VGFAMYRALL 660
KQR 663 
Gene Ontology
 GO:0031307; C:integral to mitochondrial outer membrane; ISS:UniProtKB.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0019725; P:cellular homeostasis; ISS:UniProtKB.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0097345; P:mitochondrial outer membrane permeabilization; ISS:UniProtKB.
 GO:0047497; P:mitochondrion transport along microtubule; ISS:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR013566; EF_hand_assoc_1.
 IPR013567; EF_hand_assoc_2.
 IPR002048; EF_hand_dom.
 IPR020860; MIRO.
 IPR013684; MIRO-like.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR021181; Small_GTPase_Miro. 
Pfam
 PF08355; EF_assoc_1
 PF08356; EF_assoc_2
 PF13405; EF_hand_4
 PF08477; Miro
 PF00071; Ras 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51423; MIRO 
PRINTS
 PR00449; RASTRNSFRMNG.