CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024182
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 U4/U6.U5 tri-snRNP-associated protein 1 
Protein Synonyms/Alias
 Hypoxia-associated factor; Squamous cell carcinoma antigen recognized by T-cells 1; SART-1; mSART-1 
Gene Name
 Sart1 
Gene Synonyms/Alias
 Haf 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
129ETNKLRAKLGLKPLEubiquitination[1]
336KPRSILAKYDEELEGacetylation[2]
797IVLSGSGKSMNANTIacetylation[2, 3]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. Appears to play a role in hypoxia-induced regulation of EPO gene expression. 
Sequence Annotation
 MOD_RES 430 430 Phosphothreonine (By similarity).
 MOD_RES 527 527 Phosphoserine (By similarity).
 MOD_RES 597 597 Phosphoserine (By similarity).
 MOD_RES 602 602 Phosphoserine (By similarity).
 MOD_RES 604 604 Phosphoserine (By similarity).
 MOD_RES 701 701 Phosphothreonine (By similarity).
 MOD_RES 770 770 Phosphothreonine (By similarity).  
Keyword
 Coiled coil; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Spliceosome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 806 AA 
Protein Sequence
MGSSKKHRGE KEAAGTTAAA GTGGTTEQPP RHREHKKHKH RSSGGGSSGG ERRKRSRERG 60
SERGSGRRGA EAEARSGAHG RERSQAEPSE RRVKREKRDD GYEAAASSKA SSGDASSLSI 120
EETNKLRAKL GLKPLEVNAV KKEAGTKEEP VAADVINPMA LRQREELREK LAAAKEKRLL 180
NQKLGKIKTL GEDDPWLDDT AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE 240
FEQRRQDLYS ARDLQGLTVE HAIDSFREGE TVVLTLKDKG VLQDGEDVLV NVNMVDKERA 300
DKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILAKYDEE LEGERPHSFR LEQGGMADGL 360
RERELEEIRT KLRLQAQSLS SVGPRLASEY LSPEEMVTFK KTKRRVKKIR KKEKEVIMRA 420
DDLLPLGDQT QDGDFGSRLR GRGRRRVPEV EEEALEDEEK DPVAQPPPSD DTRVENMDIS 480
DEEDGGALPP GSPEGLEEDE AELELQKQLE KGRRLRQLQQ LQQLRDSGEK VLEIVKKLES 540
RQRGWEEEED PERKGTIVFN ATSEFCRTLG EIPTYGLAGN REEQEELMDF ERDEERSANG 600
GSESDGEENI GWSTVNLDEE KQHQDFSASS TTILDEEPIV NRGLAAALLL CQNKGLLETT 660
VQKVARVKAP NKSLPSAVYC IEDKMAIDDK YSRREEYRGF TQDFKEKDGY KPDVKIEYVD 720
ETGRKLTPKE AFRQLSHRFH GKGSGKMKTE RRMKKLDEEA LLKKMSSSDT PLGTVALLQE 780
KQKAQKTPYI VLSGSGKSMN ANTITK 806 
Gene Ontology
 GO:0015030; C:Cajal body; IEA:Compara.
 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0045585; P:positive regulation of cytotoxic T cell differentiation; IEA:Compara.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR005011; SART_1. 
Pfam
 PF03343; SART-1 
SMART
  
PROSITE
  
PRINTS