UniProt Accession

 E9PEE4_HUMAN; E9PEE4 

Genbank Protein ID

 AL449212 

Genbank Nucleotide ID

  

Protein Name

 Methyl-CpG-binding domain protein 4 

Protein Synonyms/Alias

  

Gene Name

 MBD4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
56	DEEQMMIKRSSECNP	ubiquitination	[1]
117	FISPQGLKFRSKSSL	ubiquitination	[2]
397	RKDFTGEKIFQEDTI	ubiquitination	[1]
484	VLWKFLEKYPSAEVA	ubiquitination	[1, 2, 3]
504	RDVSELLKPLGLYDL	ubiquitination	[1]
526	FSDEYLTKQWKYPIE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 572 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0006284; P:base-excision repair; IEA:InterPro. 

Interpro

 IPR016177; DNA-bd_integrase-typ.
 IPR011257; DNA_glycosylase.
 IPR003265; HhH-GPD_domain.
 IPR017352; Me_CpG-bd_MBD4.
 IPR001739; Methyl_CpG_DNA-bd. 

Pfam

 PF00730; HhH-GPD
 PF01429; MBD 

SMART

 SM00391; MBD 

PROSITE

 PS50982; MBD 

PRINTS