CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007666
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Melanoma-associated antigen 1 
Protein Synonyms/Alias
 Antigen MZ2-E; Cancer/testis antigen 1.1; CT1.1; MAGE-1 antigen 
Gene Name
 MAGEA1 
Gene Synonyms/Alias
 MAGE1; MAGE1A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
105FRAVITKKVADLVGFubiquitination[1, 2, 3]
116LVGFLLLKYRAREPVubiquitination[2]
125RAREPVTKAEMLESVubiquitination[3, 4]
134EMLESVIKNYKHCFPubiquitination[4]
237SAYGEPRKLLTQDLVubiquitination[1, 2, 3, 4]
247TQDLVQEKYLEYRQVubiquitination[1, 2, 3, 4]
278LAETSYVKVLEYVIKubiquitination[1, 3]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. 
Sequence Annotation
 DOMAIN 102 301 MAGE.
 MOD_RES 57 57 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Tumor antigen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 309 AA 
Protein Sequence
MSLEQRSLHC KPEEALEAQQ EALGLVCVQA ATSSSSPLVL GTLEEVPTAG STDPPQSPQG 60
ASAFPTTINF TRQRQPSEGS SSREEEGPST SCILESLFRA VITKKVADLV GFLLLKYRAR 120
EPVTKAEMLE SVIKNYKHCF PEIFGKASES LQLVFGIDVK EADPTGHSYV LVTCLGLSYD 180
GLLGDNQIMP KTGFLIIVLV MIAMEGGHAP EEEIWEELSV MEVYDGREHS AYGEPRKLLT 240
QDLVQEKYLE YRQVPDSDPA RYEFLWGPRA LAETSYVKVL EYVIKVSARV RFFFPSLREA 300
ALREEEEGV 309 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; TAS:ProtInc.
 GO:0042826; F:histone deacetylase binding; IDA:UniProtKB.
 GO:0045746; P:negative regulation of Notch signaling pathway; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR002190; MAGE.
 IPR021072; Melanoma_ass_antigen_N. 
Pfam
 PF01454; MAGE
 PF12440; MAGE_N 
SMART
  
PROSITE
 PS50838; MAGE 
PRINTS